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Etiology

Purification and Partial Characterization of a Virus from Pineapple. U. B. Gunasinghe, Department of Plant Pathology, University of Hawaii, 3190 Maile Way, Honolulu 96822; T. L. German, Department of Plant Pathology, University of Hawaii, 3190 Maile Way, Honolulu 96822. Phytopathology 79:1337-1341. Accepted for publication 28 June 1989. Copyright 1989 The American Phytopathological Society. DOI: 10.1094/Phyto-79-1337.

Double-stranded RNA and long, flexuous, rod-shaped virus particles (1,200 × 12 nm) were recovered from mealybug wilt-affected pineapple (Ananas comosus) plants from plantations in Hawaii. A purification protocol was developed, and the virus was partially characterized. Based upon its morphology, the molecular weight of the coat protein, and analysis of double-stranded RNA, we tentatively assign this virus to the type II closterovirus group.