VIEW ARTICLE

Molecular Plant Pathology

Transformation and Cotransformation of Gaeumannomyces graminis to Phleomycin Resistance. A. L. Pilgeram, Departments of Plant Pathology and Microbiology, Montana State University, Bozeman 59717; J. M. Henson, Departments of Plant Pathology and Microbiology, Montana State University, Bozeman 59717. Phytopathology 80:1124-1129. Accepted for publication 8 May 1990. Copyright 1990 The American Phytopathological Society. DOI: 10.1094/Phyto-80-1124.

Gaeumannomyces graminis var. graminis and G. g. tritici were transformed to phleomycin resistance by an improved transformation procedure with pAN8-1, a plasmid encoding the ble gene of Streptoalloteichus hindustanus. G. g. graminis was cotransformed with pAN8-1 and pBT3, a plasmid encoding resistance to benomyl. Vector DNA apparently was integrated into the fungal genome in all transformants analyzed, at different sites in the genome and with varying copy numbers. The selected phenotypes (Phleo^R or Ben^R) were stable through mitosis in most transformants. Integrated plasmid DNA was stable through meiosis in all transformants tested.