VIEW ARTICLE

Techniques

Properties and Potential Applications of Syngeneic Monoclonal Anti-Idiotypic Antibodies: Use as a Reference Standard in Enzyme-Linked Immunosorbent Assay. R. L. Mernaugh, Department of Microbiology, Iowa State University, Ames 50011; J. H. Hill(2), D. P. Durand(3), R. A. Van Deusen(4), and K. F. Harris(5). (2)(3)Departments of Plant Pathology and Microbiology, Iowa State University, Ames; (4)Department of Plant Pathology, Iowa State University, Ames; (5)Department of Entomology, Texas A&M University, College Station 77843. Phytopathology 81:274-282. Accepted for publication 17 October 1990. Copyright 1991 The American Phytopathological Society. DOI: 10.1094/Phyto-81-274.

Syngeneic anti-idiotypic monoclonal antibodies were generated to an anti-soybean mosaic virus (SMV) monoclonal antibody. One of these antibodies (1a) was used in place of SMV to capture rabbit polyclonal anti-SMV antibodies in an enzyme-linked immunosorbent assay (ELISA). This demonstrated that an anti-idiotypic monoclonal antibody can be used as a standard in ELISA for detection of exotic pathogens when conditions preclude introduction of the pathogen into the environment.