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Etiology

Isolates of Bean Common Mosaic Virus Comprising Two Distinct Potyviruses. N. M. McKern, Principal research scientist, CSIRO, Division of Biomolecular Engineering, 343 Royal Parade, Parkville 3052, Australia; G. I. Mink(2), O. W. Barnett(3), A. Mishra(4), L. A. Whittaker(5), M. J. Silbernagel(6), C. W. Ward(7), and D. D. Shukla(8). (2)Professor, Department of Plant Pathology, Washington State University, Prosser 99350-9687; (3)Professor, Department of Plant Pathology and Physiology, Clemson University, Clemson, SC 29634-0377; (4)(5)(7)(8)Visiting scientist, Technical officer, Chief research scientist, and Senior principal research scientist, respectively, CSIRO, Division of Biomolecular Engineering, 343 Royal Parade, Parkville 3052, Australia; (4)Permanent address: Department of Plant Pathology, B.A. College of Agriculture, Gujarat Agricultural University, Anand 388110, India; (6)Research plant pathologist, USDA-ARS, Irrigated Agriculture Research and Extension Center, Prosser, WA 99350-9687. Phytopathology 82:923-929. Accepted for publication 8 June 1992. Copyright 1992 The American Phytopathological Society. DOI: 10.1094/Phyto-82-923.

Strains of bean common mosaic virus (BCMV) have been identified largely by their characteristic interactions with a selected number of differential bean cultivars. Recent studies have suggested that BCMV strains could be classified on serological and other grounds as strains of two distinct potyviruses. Using high-performance liquid chromatographic peptide profiles of coat protein digests together with limited peptide analysis, 22 isolates of BCMV, including representatives from seven recognized pathotypes, were compared with each other and with the type strains of blackeye cowpea mosaic virus (BlCMV) and peanut stripe virus (PStV). The peptide profiles confirm the existence of two distinct potyviruses among the current isolates of BCMV. BCMV strains NL-3, NL-5, NL-8, and TN-1 are strains of one of the potyviruses, which we propose should be named “bean necrosis mosaic virus,” since these strains induce temperature-insensitive necrosis in bean cultivars that carry the dominant I gene. Strains CH-2, NL-1, NL-2, NL-4, NL-6, NL-7, PR-1, RU-1, US-1, US-2 (three variant isolates), US-3, US-4 US-5, US-6, US-7, and US-10 are strains of the second potyvirus, which we suggest should retain the name BCMV. Peptide profile data also suggest that isolates of BlCMV, PStV, azuki bean mosaic virus (AzMV), and three potyvirus isolates from soybean (PM, PN, and 74) are all strains of the second potyvirus, BCMV.