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Molecular Plant Pathology

Complete Nucleotide Sequences of the Infectious Cloned DNAs of Bean Dwarf Mosaic Geminivirus. Sri H. Hidayat,Department of Plant Pathology, University of Wisconsin, Madison 53706; Robert L. Gilbertson(2), Stephen F. Hanson(3), Francisco J. Morales(4), Paul Ahlquist(5), David R. Russell(6), and Douglas P. Maxwell(7). (2)(3)(7) Department of Plant Pathology, University of Wisconsin, Madison 53706, (2)Current address: Department of Plant Pathology, University of California, Davis 95616; (4)Centro Internacional de Agricultura Tropical (CIAT), Apartado Aéreo 6713, Cali, Colombia; (5)Department of Plant Pathology and Institute for Molecular Virology, University of Wisconsin, Madison 53706; (6)Agracetus, Inc., 8520 University Green, Middleton, WI 53717. Phytopathology 83:181-187. Accepted for publication 12 October 1992. Copyright 1993 The American Phytopathological Society. DOI: 10.1094/Phyto-83-181.

Bean dwarf mosaic geminivirus (BDMV) was shown to have a bipartite genome (DNA-A and DNA-B) from restriction map and sequence data comparisons of cloned DNAs. The nucleotide sequences of the infectious clones of DNA-A and DNA-B were determined. Computer analyses showed that the bipartite genome of BDMV resembles that of other whitefly-transmitted geminiviruses. The DNA-A (2,615 nt) and DNA-B (2,575 nt) have little sequence homology other than that within the common region. The nucleotide sequences of the 187-nt common regions of the two DNAs had 96% identity and included a region of 33 nt that can form a stem-loop structure nearly identical to that of other geminiviruses. Nucleotide sequence analysis identified six open reading frames, four on DNA-A and two on DNA-B, involving viral and complementary strand sense transcription. Comparisons of nucleotide and derived amino acid sequences of BDMV with those of other whitefly-transmitted geminiviruses showed that BDMV was most closely related to Abutilon mosaic geminivirus and not closely related to bean golden mosaic geminivirus. BDMV can be distinguished from other bean-infecting geminiviruses by differences in symptoms, host range, reaction of bean germplasm, and sequence identities; thus, BDMV is a distinct bean-infecting geminivirus.