VIEW ARTICLE

Resistance

Inheritance of the Resistance to Macrophomina phaseolina and Identification of RAPD Markers Linked to the Resistance Genes in Beans. G. Olaya, former graduate research assistant and professor, respectively, Department of Plant Pathology, Cornell University, New York State Agricultural Experiment Station, Geneva 14456-0462; G. S. Abawi(2), and N. F. Weeden(3). (2)former graduate research assistant and professor, respectively, Department of Plant Pathology; (3)associate professor, Department of Horticultural Sciences, Cornell University, New York State Agricultural Experiment Station, Geneva 14456-0462. Phytopathology 86:674-679. Accepted for publication 28 March 1996. Copyright 1996 The American Phytopathological Society. DOI: 10.1094/Phyto-86-674.

The inheritance of resistance to Macrophomina phaseolina was determined using traditional approaches and molecular markers. Inheritance studies were based on a cross between the resistant accession BAT-477 and the susceptible accession A-70. Resistance to M. phaseolina was examined by inoculating bean seeds with soil infested with sclerotia of M. phaseolina. The ratio of resistant to susceptible plants in the F₂ population was close to 9:7. Bulked segregant analysis identified two random amplified polymorphic DNA (RAPD) markers linked to M. phaseolina resistance. The first RAPD marker (B459₁₆₀₀) was a 1.6-kb band present in the susceptible and absent in the resistant plants. The second RAPD marker (B386₉₀₀) was a 0.9-kb band present in the resistant, but absent in the susceptible plants. Each of the RAPD markers segregated in a 3:1 ratio as expected, and the segregation of both markers followed a 9:3:3:1 ratio expected for two unlinked loci. In 15 of the 16 F₃ families examined, the genotype of the markers correctly predicted the genotype (heterozygous or homozygous) for disease resistance. The one exception could be attributed to recombination between B459₁₆₀₀ and the linked susceptible gene. These data were consistent with the hypothesis that, in BAT-477, resistance to M. phaseolina is controlled by two dominant complementary genes. The symbols Mp-1 and Mp-2 (Macrophomina phaseolina) were proposed for these genes.