Procedures for Growth and Inoculation of Xanthomonas fragariae, Causal Organism of Angular Leaf Spot of Strawberry. W. J. Hazel, Fruit Laboratory, Horticultural Science Institute, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, Beltsville, MD 20705. E. L. Civerolo, Fruit Laboratory, Horticultural Science Institute, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, Beltsville, MD 20705; and G. A. Bean, Botany Department, University of Maryland, College Park 20742. Plant Dis. 64:178-181. Accepted for publication 1 October 1979. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1980. DOI: 10.1094/PD-64-178.

Growth of Xanthomonas fragariae, the cause of strawberry leaf spot, was optimal in 0.8% nutrient broth, 0.5% casein hydrolysate, 0.1% yeast extract, 1% glucose, and minerals. Distilled, demineralized water and saline (0.85% NaCl) were satisfactory inoculum diluents. Spray infiltration was the most rapid, sensitive, and reliable method of leaf inoculation. The number of lesions increased linearly with inoculum titer and as leaf age decreased. The number of lesions per 0.8-cm² site ranged from 12.04 to 102.44.