Enzyme-Linked Immunosorbent Assay to Detect Potato Leafroll Virus in Potato Tubers and Viruliferous Aphids. R. G. Clarke, Assistant Professor, Department of Entomology, Oregon State University, Corvallis, OR 97331. R. H. Converse, Research Plant Pathologist, Agricultural Research, SEA, U.S. Department of Agriculture, and Oregon State University; and M. Kojima, Associate Professor, Faculty of Agriculture, Niigata University, Niigata, Japan. Plant Dis. 64:43-45. Accepted for publication 21 March 1979. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1980. DOI: 10.1094/PD-64-43.

Enzyme-linked immunosorbent assay (ELISA) with alkaline phosphatase successfully detected potato leafroll virus (PLRV) in naturally infected Russet Burbank potato tubers. Statistically significant differences in A₄₀₅ values of ELISA reactions could be detected between known infected and healthy tuber samples. A multiple-eye sampling scheme was devised to detect PLRV in potato tubers by the ELISA method. PLRV was also detected in viruliferous Myzus persicae by the ELISA method.