Multiple Inoculation Technique for Evaluating Resistance of Single Barley Seedlings to Three Fungi. R. A. Kilpatrick, Research Plant Pathologist, Field Crops Laboratory, Plant Genetics and Germplasm Institute, U.S. Department of Agriculture, Science and Education Administration, Agricultural Research, Beltsville MD 20705. P. S. Baenziger, Research Geneticist, J. G. Moseman, Institute Chairman, Field Crops Laboratory, Plant Genetics and Germplasm Institute, U.S. Department of Agriculture, Science and Education Administration, Agricultural Research, Beltsville MD 20705. Plant Dis. 65:504-506. . This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1981. DOI: 10.1094/PD-65-504.

A multiple inoculation technique was developed for evaluating resistance of barley seedlings to Rhynchosporium secalis, Puccinia hordei, and Erysiphe graminis hordei. Differences in disease reaction were highly significant among cultivars inoculated with the three fungi. Disease reaction in the multiple inoculation test correlated well with disease reaction in concurrent single inoculation tests. The technique should prove valuable for evaluating barley seedlings where seed supplies or space are limited and results are needed rapidly.