Identification of Turnip Mosaic and Cauliflower Mosaic Viruses Naturally Infecting Collards. Mushtaq A. Khan, Visiting Post-Doctoral Associate, Department of Plant Pathology, University of Georgia Experiment Stations, Georgia Station, Experiment 30212. J. W. Demski, Associate Professor, Department of Plant Pathology, University of Georgia Experiment Stations, Georgia Station, Experiment 30212. Plant Dis. 66:253-256. Accepted for publication 4 December 1981. Copyright 1982 American Phytopathological Society. DOI: 10.1094/PD-66-253.

Two viruses, cauliflower mosaic (CaMV) and turnip mosaic (TuMV), isolated from naturally infected collards, were identified by host range, physical properties, inclusion bodies, and serology. Prominent vein chlorosis and banding were associated with CaMV infections. TuMV was isolated from collards with very mild mottle and small chlorotic ring spots to severe mosaic and blisters. The collard isolates of TuMV differed from the type isolate in that they infected legumes (Lupinus albus, L. angustifolius, Pisum sativum, and Phaseolus vulgaris ‘Bountiful’). The accuracy of two serodiagnostic tests for detecting TuMV was compared with that of bioassays. Latex agglutination serologic tests were more sensitive than sodium lauryl sulfate agar diffusion serologic tests and were useful in detecting TuMV from field plant samples of collards, turnips, and mustard. Inspection for virus-induced inclusions was a useful tool for diagnosing CaMV infection in collards but was less effective for TuMV.

Keyword(s): Brassica oleracea var. acephala, legume strain of turnip mosaic virus.