Growth and Sporulation of Botryodiplodia hypodermia in Response to Different Agar Media and Temperatures. J. M. Krupinsky, Plant Pathologist, ARS, USDA, Northern Great Plains Research Center, Mandan, ND 58554. Plant Dis. 66:481-483. Accepted for publication 13 August 1981. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1982. DOI: 10.1094/PD-66-481.

Growth and spore production of Botryodiplodia hypodermia were determined on different culture media. The effects of temperature and light on fungal growth and spore production were evaluated. Three isolates of B. hypodermia could not be consistently differentiated from one another in these studies. There was a significant interaction between temperature and medium in three temperature studies. Growth and spore production were greatest on Difco potato-dextrose agar and “homemade” potato-dextrose agar, followed by yeast malt extract agar and V-8 juice agar. Growth and spore production were best at 25 ± 1 C on the potato-dextrose agars, but 21 ± 1 C was the optimum temperature on V-8 juice agar. Sterile wheat kernels added to the surface of these media promoted additional growth and conidial production and provided a convenient source of inoculum.

Keyword(s): Siberian elm, Ulmus pumila.