Diagnosis of Rice Ragged Stunt Virus by Enzyme-Linked Immunosorbent Assay and Immunosorbent Electron Microscopy. E. Luisoni, Istituto di Fitovirologia Applicata del C.N.R., Via Vigliani 104, 10135 Torino, Italy. R. G. Milne, and P. Roggero, Istituto di Fitovirologia Applicata del C.N.R., Via Vigliani 104, 10135 Torino, Italy. Plant Dis. 66:929-932. Accepted for publication 11 February 1982. Copyright 1982 American Phytopathological Society. DOI: 10.1094/PD-66-929.

Rice ragged stunt virus was purified and specific antisera were prepared that reacted only with the virus capsid and not with double-stranded ribonucleic acid. Enzyme-linked immunosorbent assay and immunosorbent electron microscopy detected the virus in infected rice plants. Immunosorbent electron microscopy was from 10 to more than 100 times more sensitive, but enzyme-linked immunosorbent assay is likely to find wider local application because it requires simpler, less costly equipment.

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