A Technique for Quantitative Use of Nematodes from Monoxenic Tissue Culture as Soil Inoculum. M. J. Martin, Research Associate, Department of Plant Pathology, Oklahoma State University, Stillwater 74078. R. M. Riedel, Professor, Department of Plant Pathology, Ohio State University, Columbus 43210; and R. C. Rowe, Associate Professor, Department of Plant Pathology, Ohio Agricultural Research and Development Center, Wooster 44691. Plant Dis. 67:275-277. Accepted for publication 2 August 1982. Copyright 1983 American Phytopathological Society. DOI: 10.1094/PD-67-275.

A technique for using monoxenic cultures of Pratylenchus penetrans, P. scribneri, and P. crenatus directly as soil inoculum is discussed. The technique has been used successfully to infest both organic and mineral soils. The technique can be used to infest large quantities of soil for use in field microplots. Practical applications, advantages, and disadvantages of the technique are discussed.