An Inoculation Technique to Detect the HtN Gene in Inbred Lines of Corn Under Greenhouse Conditions. S. Leath, Graduate Research Assistant, Department of Plant Pathology, University of Illinois, Urbana-Champaign 61801. W. L. Pedersen, Assistant Professor, Department of Plant Pathology, University of Illinois, Urbana-Champaign 61801. Plant Dis. 67:520-522. Accepted for publication 27 September 1982. Copyright 1983 American Phytopathological Society. DOI: 10.1094/PD-67-520.

A 10-μl drop of inoculum containing about 500 conidia (50,000 conidia per milliliter) of Exserohilum turcicum was applied to the leaf surfaces of 11-day-old corn (Zea mays) seedlings. After 10 days, susceptible inbred lines could be distinguished from lines with either of the dominant resistance genes Ht1 or HtN based on incubation periods and lesion characteristics. This technique was also effective in determining the portion of an inbred population carrying the HtN gene.

Keyword(s): northern corn leaf blight.