Detection of 41 Isolates of Necrotic Ringspot, Apple Mosaic, and Prune Dwarf Viruses in Prunus and Malus by Enzyme-Linked Immunosorbent Assay. J. P. McMorran, Research Assistant Unclassified, Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331. H. R. Cameron, Professor, Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331. Plant Dis. 67:536-538. Accepted for publication 29 October 1982. Copyright 1983 American Phytopathological Society. DOI: 10.1094/PD-67-536.

Enzyme-linked immunosorbent assay (ELISA) detected 41 isolates of ilarvirus in Prunus and Malus representing the entire symptomatic and serological range of necrotic ringspot virus (NRSV), apple mosaic virus (ApMV), and prune dwarf virus (PDV). Three isolates reported to be NRSV were not detected. Of 11 antisera used, only NRSV-G, RMV, PDV-B were required to detect all 41 isolates. These three antisera, which were commercially available, were combined to detect any of the 41 isolates in singular assays. Results of a comparative bioassay using Chenopodium quinoa and Cucumis sativus are discussed.