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Research A High Humidity Incubation Chamber for Foliar Pathogens. J. M. Krupinsky, Plant Pathologist, USDA, ARS, Northern Great Plains Research Center, P.O. Box 459, Mandan ND 58554. A. L. Scharen, Plant Pathologist, USDA, ARS, Department of Plant Pathology, Montana State University, Bozeman 59717. Plant Dis. 67:84-86. Accepted for publication 30 May 1982. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1983. DOI: 10.1094/PD-67-84. A chamber used to create and maintain a high humidity atmosphere for inoculations with foliar pathogens of plants is described. The chamber was constructed from polyvinyl chloride pipe, which is inert and rustproof. The clear polyethylene plastic cover on the chamber was replaced easily whenever necessary. Humid air piped into the chamber from two commercial cold-water humidifiers was evenly distributed along the sides of the chamber to provide uniform high humidity throughout the chamber. Relative humidity levels of 99 and 100% were maintained at various temperatures ranging from 14 to 36 C inside the chamber. |