A Technique for Isolating the Mycorrhizal Fungus Laccaria laccata from Conifer Seedlings. J. M. Genua, Former Project Assistant, Department of Plant Pathology, Pennsylvania State University, University Park 16802. L. C. Schisler and W. Merrill, Professors, Department of Plant Pathology, Pennsylvania State University, University Park 16802. Plant Dis. 69:1080-1081. Accepted for publication 28 May 1985. Copyright 1985 The American Phytopathological Society. DOI: 10.1094/PD-69-1080.

A protocol involving an extensive washing sequence and modified Melin-Norkrans’ medium containing gentamicin sulfate, achromycin V, and benomyl was developed for isolating the mycorrhizal fungus Laccaria laccata from greenhouse-and field-grown conifer seedlings. Isolation rates of 54 and 76% were attained from field-grown seedlings of Pinus resinosa and Pseudotsuga menziesii, respectively.