Detection of Potato Viruses S, X, and Y by Enzyme-Linked Immunosorbent Assay on Nitrocellulose Membranes (Dot-ELISA). E. E. Banttari, Professor, Department of Plant Pathology, University of Minnesota, St. Paul 55108. P. H. Goodwin, Research Assistant, Department of Plant Pathology, University of Minnesota, St. Paul 55108. Plant Dis. 69:202-205. Accepted for publication 15 October 1984. Copyright 1985 The American Phytopathological Society. DOI: 10.1094/PD-69-202.

Potato viruses S, X, and Y were detected by enzyme-linked immunosorbent assay (ELISA) on nitrocellulose membranes at dilutions of 1:16,000, 1:128,000, and 1:16,000, respectively (parts infected potato sap:parts healthy potato sap). The assay for potato virus X was at least eight times more sensitive than double-antibody sandwich ELISA (DAS-ELISA) in polystyrene cuvettes. DAS-ELISA was used with alkaline phosphatase conjugates of each gamma globulin and a 0.45-μm pore size nitrocellulose membrane was used as the solid phase. Naphthol AS-MX phosphate and fast red TR salt were used as the substrate and stain that produced visually detectable 'dots' on the membranes. Quantitative data on reaction intensities were obtained with a reflectance densitometer. This ELISA modification is termed dot-ELISA.

Keyword(s): immunoblot assay.