Use of Cellulose Acetate Electrophoresis as an Alternative to Starch Gel Electrophoresis for Detecting Root-Knot Nematode Resistance in Tomato. H. A. Bolkan, Senior Scientist, Campbell Institute for Research and Technology, Route 1, Box 1314, Davis, CA 95616. Valerie M. Williamson, and C. M. Waters. Senior Research Scientist, Plant Cell Research Institute, 6560 Trinity Court, Dublin, CA 94568; and Research Associate, Campbell Institute for Research and Technology, Davis, CA. Plant Dis. 71:1001-1003. Accepted for publication 9 March 1987. Copyright 1987 The American Phytopathological Society. DOI: 10.1094/PD-71-1001.

Detection of the acid phosphatase-1 (Aps-1) isozyme from tomato genotypes susceptible, homozygous resistant, and heterozygous resistant to root-knot nematode was compared by cellulose acetate and starch gel electrophoresis. In contrast to starch gel electrophoresis, cellulose acetate electrophoresis made discrete separations of Aps-1 bands and gave better resolution. In all tests, cellulose acetate electrophoresis was quicker and more economical than starch gel electrophoresis in assaying for the Aps-1 isozyme.