Parallel Identification of Five Luteoviruses That Cause Barley Yellow Dwarf. W. F. Rochow, Research Plant Pathologist, Agricultural Research Service, U.S. Department of Agriculture, and Professor, Cornell University, Ithaca, NY 14853. J. S. Hu, R. L. Forster, and H. T. Hsu. Graduate Research Assistant, Department of Plant Pathology, Cornell University, Ithaca, NY 14853; Associate Professor of Plant Pathology, University of Idaho, Research and Extension Center, Kimberly 83341; and Research Microbiologist, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705. Plant Dis. 71:272-275. Accepted for publication 25 August 1986. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1987. DOI: 10.1094/PD-71-0272.

A modified, indirect enzyme-linked immunosorbent assay (EIA) was tested for SGV to permit simultaneous use with direct EIA for four other luteoviruses (RPV, RMV, PAV, and MAV) that also cause barley yellow dwarf. Specificity and reliability of the method were shown during 15 mo in tests of both clarified and purified preparations of the five viruses and of samples of grains and grasses from the field. The comparative assay was especially useful in study of five SGV-like viruses from Idaho that had a range of biological properties. For the sixth consecutive year, most samples collected in New York contained viruses similar to PAV, and none had virus similar to MAV.

Keyword(s): aphid vectors, monoclonal antibodies.