Evaluation of Wheat Germ Plasm for Resistance to Wheat Streak Mosaic Virus by Symptomatology, ELISA, and Slot-Blot Hybridization. S. L. Stoddard, Former Graduate Research Assistant, Department of Plant Pathology, Throckmorton Hall, Kansas State University, Manhattan 66506. S. A. Lommel, and B. S. Gill. Assistant Professor, and Associate Professor, Department of Plant Pathology, Throckmorton Hall, Kansas State University, Manhattan 66506. Plant Dis. 71:714-719. Accepted for publication 13 March 1987. Copyright 1987 The American Phytopathological Society. DOI: 10.1094/PD-71-0714.

Enzyme-linked immunosorbent assay (ELISA) and a slot-blot hybridization assay were developed to evaluate a large number of accessions of wild wheat species, in conjunction with a symptomatology assay, for resistance to wheat streak mosaic virus (WSMV). No resistance was found among the Triticum or the Aegilops species tested. Five wheat/wheatgrass (Thinopyrum = Agropyron species) amphiploids were found resistant to WSMV. Nine registered germ plasms resistant to WSMV were evaluated; eight were immune to the virus and one gave a susceptible reaction. Both ELISA and slot-blot hybridization assays were sensitive in virus detection. The three assays showed good correlation.