Partial Characterization and Serological Analysis of Pseudo-Curly Top Virus. L. L. McDaniel, University of Florida, IFAS, Fort Lauderdale Research and Education Center, Fort Lauderdale 33314. J. H. Tsai, University of Florida, IFAS, Fort Lauderdale Research and Education Center, Fort Lauderdale 33314. Plant Dis. 74:17-21. Accepted for publication 3 July 1989. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1990. DOI: 10.1094/PD-74-0017.

A polyclonal antiserum was prepared to partially purified virions of pseudo-curly top virus (PCTV), a provisional member of the geminivirus group. The PCTV virions were detected by double antibody sandwich enzyme-linked immunosorbent assay in Lactuca sativa, Solanum melongena, S. nigrum, and Lycopersicon esculentum, as well as in the treehopper vector Micrutalis malleifera. Indirect ELISA tests showed a relationship between PCTV and beet curly top virus. Nucleic acid extracted from partially purified PCTV virions was digested by DNase I but not by RNase A.