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Disease Note. A Selective Medium for Gaeumannomyces-like Fungi. M. L. Elliott, University of Florida, IFAS, Fort Lauderdale Research and Education Center, Fort Lauderdale 33314. Plant Dis. 75:1075. Accepted for publication 21 June 1991. Copyright 1991 The American Phytopathological Society. DOI: 10.1094/PD-75-1075A. The selective medium SM-GGT7 for Gaeumannomyces spp.,
Phialophora spp., and Magnaporthe poae has been developed to
replace SM-GGT3, the medium previously used for Gaeumannomyces-like
fungi (2). SM-GGT7 also replaces the previously reported medium
SM-GGT4 (1). SM-GGT7 contains 39 g of Difco PDA, 100 mg of
streptomycin, 10 mg of dichloran, 10 mg of meta1axyl, 50 mg of
vinclozolin, 1 mg of CGA-173506, and 500 mg of L-dopa per liter
of deionized water; amounts given are for active ingredients. A sample
of CGA-173506, a phenylpyrrole, may be obtained from Vince Morton
(CIBA-GEIGY Corp., Agricultural Division, P.O. Box 18300, Greensboro,
NC 27419). The antibiotics and fungicides are added to sterile
molten PDA cooled to 50 C. In comparisons of isolation efficacy
using turfgrass field samples with root rot symptoms typically caused
by Gaeumannomyces-like fungi, SM-GGT7 was as effective as SMGGT3
in isolating these fungi from plant tissue. |