Survey and Detection of Endophytic Fungi in Lolium Germ Plasm by Direct Staining and Aphid Assays. A. Dan Wilson, Postdoctoral Research Associate, Plant Germplasm Introduction and Testing Research Unit, USDA-ARS, Washington State University, Pullman 99164-6402. Stephen L. Clement, and Walter J. Kaiser. Research Entomologist, and Research Plant Pathologist, Plant Germplasm Introduction and Testing Research Unit, USDA-ARS, Washington State University, Pullman 99164-6402. Plant Dis. 75:169-173. Accepted for publication 26 July 1990. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1991. DOI: 10.1094/PD-75-0169.

Clavicipitaceous anamorphic endophytes were detected in 28 of 85 accessions from five of eight species in a collection of Lolium germ plasm. Comparative descriptions of endophytic mycelium in seeds of L. multiflorum, L. perenne, L. persicum, L. rigidum, and L. temulentum revealed morphological characteristics distinguishing endophytes in each host species. Most endophyte-infected accessions originated from Eurasia, particularly from Middle Eastern countries. Endophyte incidence and infection percentages varied widely with host species and geographic origin. Feeding tests with Diuraphis noxia were useful in detecting endophyte-infected accessions of perennial ryegrass.

Keyword(s): Acremonium, bioassay, biological control, Clavicipitaceae, Russian wheat aphid.