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Disease Note Identification of Carrot Thin Leaf Virus in California Carrots. B. W. Falk, Department of Plant Pathology, University of California, Davis 95616. R. M. Davis, and M. Piechocki. Department of Plant Pathology, University of California, Davis 95616. Plant Dis. 75:319. Accepted for publication 22 October 1990. Copyright 1991 The American Phytopathological Society. DOI: 10.1094/PD-75-0319C. A disease of carrots in California was shown to be caused by carrot
thin leaf virus (CA-CTLV). CA-CTLV was recovered only from carrots
grown in the San Joaquin Valley and not from carrots collected from
the Salinas or Imperial Valley carrot-producing areas. CA-CTLV was
transmitted by both Myzus persicae and Cavariella aegopodii in a
nonpersistent manner. Purified virions showed typical potyvirus
morphology when examined by electron microscopy, and a capsid
protein Mr of approximately 33,000 was determined by SDS-PAGE.
Antisera produced to CA-CTLV reacted positively with extracts of
plants infected with CA-CTLV. Nearly identical reactions were
obtained when CA-CTLV and the Washington (WA) CTLV isolate
(1) were compared in immunoblots using both CA-CTLV and
W A-CTLV antigens and antisera, showing that CA-CTLV is closely
related serologically to W A-CTLV. |