Characterization of Cucumber Mosaic Virus Isolates from California. J. Daniels, Former Graduate Student, Department of Plant Pathology, University of California, Davis 95616. R. N. Campbell, Professor, Department of Plant Pathology, University of California, Davis 95616. Plant Dis. 76:1245-1250. Accepted for publication 29 July 1992. Copyright 1992 The American Phytopathological Society. DOI: 10.1094/PD-76-1245.

Thirty cucumber mosaic virus (CMV) isolates from California were characterized biologically (host reaction and thermosensitivity), serologically (protein A sandwich-enzyme-linked immunosorbent assay [PAS-ELISA]) and physically (viral capsid protein migration in polyacrylamide gel electrophoresis [PAGE], peptide mapping, and migration of viral double-stranded RNAs [dsRNAs] in PAGE). All methods supported the classification of CMV isolates into two main subgroups, CMV-I and CMV-II. The subgroup CMV-I was divided further into CMV-Ia and CMV-Ib on the basis of host reaction and dsRNA patterns. A low molecular weight RNA 5, presumably a satellite RNA, was detected in two isolates. Peanut stunt virus, tomato aspermy virus, and CMV were distinguished from each other by PAS-ELISA, peptide mapping, and host reaction.