Mechanical Transmission, Susceptibility, and Host Response in Bing Sweet Cherry and Three Rootstocks by the Walnut Strain of Cherry Leafroll Virus. Adib Rowhani, Assistant Plant Pathology Specialist and Research Plant Pathologist, Agricultural Research Service, U.S. Department of Agriculture, Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis 95616. S. M. Mircetich, Assistant Plant Pathology Specialist and Research Plant Pathologist, Agricultural Research Service, U.S. Department of Agriculture, Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis 95616. Plant Dis. 76:264-266. Accepted for publication 1 October 1991. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1992. DOI: 10.1094/PD-76-0264.

A purified preparation of the walnut isolate of cherry leafroll virus (CLRV) was successfully transmitted to cherry (Prunus avium cv. Bing), to seedling Mazzard rootstock (P. avium), and to Mahaleb rootstock (P. mahaleb) but not to rooted cuttings of Colt rootstock (P. avium × P. pseudocerasus). CLRV-W infection produced a characteristic pitting symptom in the woody cylinder of susceptible cultivars. Its presence was confirmed by enzyme-linked immunosorbent assay in extracts prepared from symptomatic leaves or bark tissues. Extracts of Colt were uniformly negative, as were tests from buffer-inoculated control tissues.