Production of Monoclonal Antibodies of Beet Western Yellows Virus and Potato Leafroll Virus and Their Use in Luteovirus Detection. P. J. Ellis, Agriculture Canada, Research Station, 6660 N.W. Marine Drive, Vancouver, British Columbia, Canada V6T 1X2. A. Wieczorek, Agriculture Canada, Research Station, 6660 N.W. Marine Drive, Vancouver, British Columbia, Canada V6T 1X2. Plant Dis. 76:75-78. Accepted for publication 7 August 1991. Copyright 1992 The American Phytopathological Society. DOI: 10.1094/PD-76-0075.

Mouse line RBF/Dn immunized with beet western yellows virus (BWYV) resulted in two hybridoma clones secreting BWYV-specific antibodies. Another mouse line (BALB/c) immunized with a combination of BWYV and potato leafroll virus (PLRV) resulted in 17 hybridomas that tested positive for either BWYV or PLRV and one that reacted with an epitope common to both viruses. Selected monoclonal antibodies specific to either BWYV or PLRV were used in ELISA tests to detect luteovirus strains and isolates. Isolates of beet mild yellowing virus, turnip yellows virus, and the RPV (Rhopalosiphum padi-transmitted) strain of BYDV were closely related to BWYV, whereas solanum yellows virus and four isolates from potato were identified as PLRV. None of the monoclonals reacted with healthy plant sap.