Rapid Identification of Xanthomonas fragariae in Infected Strawberry Leaves -by Enzyme-Linked Immunosorbent Assay. A. ROWHANI, Department of Plant Pathology, University of California, Davis 95616. A. J. FELICIANO, T. LIPS, and W. D. GUBLER, Department of Plant Pathology, University of California, Davis 95616. Plant Dis. 78:248-250. Accepted for publication 1 November 1993. Copyright 1994 The American Phytopathological Society. DOI: 10.1094/PD-78-0248.

An indirect enzyme-linked immunosorbent assay using the F(ab')2 fragment as a trapping antibody for Xanlhomonas fragariae was developed. The pathogen was detected in extracts of single 4-mm leaf disks containing a 4- to 5-day-old lesion obtained by artificial inoculation or lesions in field collections from plants showing symptoms of angular leaf spot disease. Our antiserum reacted positively with 34 strains of X. fragariae but not with 16 of 17 pathovars of X. campestris and strains of Agrobacterium spp., Erwinia amylovora, Pseudomonas spp., Rhodococcus fascians. and nonpathogenic bacteria isolated from strawberry plants.

Keyword(s): crown rot, Fragaria X ananassa Duchesne, vascular collapse