Serological Detection of Onion Yellow Dwarf Virus in Garlic. MARGERY KOCH, Vegetable Crops Department, Department, Agricultural Research Organization, P.O. Box 6, Bet Dagan, 50250, Israel. R. SALOMON, Virology Department, Agricultural Research Organization, P.O. Box 6, Bet Dagan, 50250, Israel. Plant Dis. 78:785-788. Acceplcd for publication 14 March 1994. Copyright 1994 The American Phytopathological Society. DOI: 10.1094/PD-78-0785.

A highly sensitive antigen-coated plate enzyme-linked immunosorbent assay (ACP-ELISA) was developed to test for potyvirus in garlic leaves. Antiserum adsorbed with an equal volume of sap from healthy leaves is routinely used at 1:10,000 dilution and is able to detect virus at dilutions up to 1:40,000. Leaf sap can be diluted up to 1:512 when using antibody at the routine concentration. Virus was detected in plants 2 wk after inoculation via aphids. Immunosorbent electron microscopy showed that the potyvirus being tested was only onion yellow dwarf virus (OYDV). All field-grown plants tested were OYDV-infected.