Beet Necrotic Yellow Vein Virus and Its Relationship to Eight Sugar Beet Furo-like Viruses from the United States. G. C. WISLER, USDA/ARS, 1636 E. Alisal St., Salinas, CA 93905. H.-Y. LIU, and J. E. DUFFUS, USDA/ARS, 1636 E. Alisal St., Salinas, CA 93905. Plant Dis. 78:995-1001. Accepted for publication 19 July 1994. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society. 1994. DOI: 10.1094/PD-78-0995.

The degree of related ness among five beet necrotic yellow vein virus (BNYVV) isolates and eight other rigid, rod-shaped viruses coined beet soilborne mosaic virus (BSBMV) isolated from sugar beet roots from the United States was evaluated by serology, electron microscopy, fungal transmission, the polymerase chain reaction (PCR), and host range. Polyclonal antisera to the C-terminal 60 amino acids of the BNYVV coat protein (CP), the 14- and 75-kDa nonstructural proteins, and seven monoclonal antibodies were specific to BNYVV in Western blots. Antisera to the BNYVV CP and its cloned CP reacted strongly with the 22-kDa CP of the BNYVV isolates, but weakly with the 24-kDa CP of the BSBMV. Antisera to the 42-kDa BNYVV nonstruclural protein reacted with a 42-kDa protein of all BNYVV isolates and with a 44-kDa protein of all but one BSBMV isolate. No cross-reactivity was observed in reciprocal immunodiffusion tests between BNYVV and the RSBMV isolates using antisera to the CP of each virus. No products were observed for the BSBMV isolates analy7ed in PCR using 10 BNYVV primer pairs. The eight BSBMV isolates investigated induced symptoms different from those of BNYVV in several hosts. Two BSBMV isolates tested were transmitted by Polymyxa hetae. These eight BSBMV isolates appear to be furoviruscs distinct from BNYVV.

Keyword(s): Beta vulgaris, rhizomania, soilborne virus, fungal vector