Disease Note.

New Hosts of Tomato Spotted Wilt Virus. C. Jorda, Department Vegetal Production, Plant Pathology (Agronomos), Universidad Politecnica. Cno. de Vera 14, Valencia, Spain. M. Juarez. Department Vegetal Production, Plant Pathology (Agronomos), Universidad Politecnica. Cno. de Vera 14, Valencia, Spain. Plant Dis. 79:538. Accepted for publication 10 October 1994. Copyright 1995 The American Phytopathological Society. DOI: 10.1094/PD-79-0538B.

Tomato spotted wilt tospovirus (TSWV) causes epidemics in many crops through the Mediterranean region. Tomato, pepper, and lettuce are the most affected crops in Spain. To identify hosts in areas where the virus is prevalent, 65 species of plants were tested for TSWV by double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) with commercial antisera (Loewe Biochemica, Germany: BR-01 [sero-group I or TSWV-L], BR-03 [serogroup II type I], SA-05 [serogroup type II], TSW Antiserum, and Impatiens necrotic spot tospovirus [INSV] Antiserum). Thirty-two species were found to be infected, and nine of them are new hosts of TSWV (1,2). Two are the ornamental plants Li-mnnium sinuatum (L.) Mill. (Statice), and Moruccella laevis L. (Moru-cela). Seven weed species were identified as hosts: Amaranthus blitum L., Cynodon dactilon L., Link, Galium tricornutum Dandy, G. parisiensis L., Lamium amplexicaule L., Silene vulgaris (Moench) Garcke, and Son-chus tenerrimus L. Most were found to be positive in DAS-ELISA with antiserum to defeat serogroup I isolates. All tests carried out with antiserum to defeat INSV were negative.