Host Range and Sequence Analysis of an Isolate of Potato Virus Y Inducing Veinal Necrosis in Pepper. L. d'AQUINO, Agricultural Biotechnology Center, Institute for Plant Sciences, H-2101 Godollo, Hungary. T. DALMAY, and J. BURGYAN, Agricultural Biotechnology Center, Institute for Plant Sciences, H-2101 Godollo, Hungary; and A. RAGOZZINO and F. SCALA, Istituto di Patologia Vegetale, Universita degli Studi di Napoli, 80055 Portici, Italy. Plant Dis. 79:1046-1050. Accepted for publication .1 January 1995. Copyright 1995 The American Phytopathological Society. DOI: 10.1094/PD-79-1046.

An isolate of potato virus Y (PVY), obtained from pepper (Capsicum annuum L.) showing veinal necrosis and named PVY-nnp, was compared with other PVY isolates for host range. In addition, the 3' noncoding region (.V-NCR) and coat protein gene (CP-gene) of PVY-nnp were cloned, sequenced, and aligned with the same regions of other PVY isolates. Biological characterization showed that PVY-nnp differs from all other PVY strains including isolates commonly infecting pepper. The nucleotide sequence comparison of PVY-nnp with other PVY isolates exhibited a homology ranging from 85.5 to 99.7% for the 3'-NCR, and from 87.5 to 94% for the CP-gene. The deduced amino acid sequence of the CP had a similarity ranging from 94.7 to 97.3%. These results indicate that PVY-nnp is a PVY isolate in spite of its peculiar biological properties.

Keyword(s): PVY coat protein sequence, PVY 3' noncoding region sequence, PVY taxonomy