Isozyme Analysis of Colletotrichum gloeosporioides from Five Host Genera. P. J. Kaufmann, Graduate Assistant; Department of Plant Pathology, University of Arkansas, Fayetteville 72701. G. J. Weidemann, Professor, Department of Plant Pathology, University of Arkansas, Fayetteville 72701. Plant Dis. 80:1289-1293. Accepted for publication 16 August 1996. Copy right 1996 The American Phytopathological Society. DOI: 10.1094/PD-80-1289.

Thirty-three isolates of Colletotrichum gloeosporioides isolated from five plant genera were characterized using isozyme electrophoresis. Using 11 selected enzymes, 148 electrophoretic phenotypes were identified among the 33 isolates examined. Nicotinamide adenine dinucleotide dehydrogenase (NADHDH) and diaphorase (DIA) yielded the greatest numbers of electrophoretic phenotypes. Three major groups (I, II, and III) and four subgroups (IA, IB, IIIA, and IIIB) were delineated within C. gloeosporioides among the five host genera following a cluster analysis of electrophoretic phenotype values for the 11 enzymes. With the exception of one isolate from citrus, C. gloeosporioides isolates clustered on the basis of host origin. This study suggests that isozyme analysis may be a useful tool for characterizing intraspecific population diversity within C. gloeosporioides.

Keyword(s): Aeschynomene, Carya, Citrus, Glomerella cingulata, Malva, Stylosanthes