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Research. PCR Assay for Detection of the Phytoplasma Associated with Maize Bushy Stunt Disease. N. A. Harrison, University of Florida, IFAS, Fort Lauderdale Research & Edu cation Center, 3205 College Ave., Fort Lauderdale 33314. P. A. Richardson, and J. H. Tsai, University of Florida, IFAS, Fort Lauderdale Research & Education Center, 3205 College Ave., Fort Lauderdale 33314; M. A. Ebbert, Department of Entomology, OARDC, The Ohio State University, Wooster 44691; and J. B. Kramer, University of Florida, Tropical Research and Education Center, 19805, S.W. 280 St., Homestead 33031. Plant Dis 80:263. Accepted for publication 30 November 1995. Copyright 1996 The American Phytopathological Society. DOI: 10.1094/PD-80-0263. DNA amplification by polymerase chain reaction (PCR) was used to detect the phytoplasma associated with maize bushy stunt (MBS) disease. A pair of oligonucleotide primers was synthesized according to partial sequences of a cloned 1-kb fragment of genomic DNA of the MBS phytoplasma ( |