Advances on CRISPR-based pathogen detection
Broadcast Date: April 29, 2024 | 12:00pm - 1:30pm CDT
Clustered regularly interspaced short palindromic repeats (CRISPR, pronounced “crisper”) were first detected in 1987 by Yoshizumi Ishino in Escherichia coli. While at the time, the lack of sufficient DNA sequence data made it impossible to predict their function, advances on sequencing technologies and further studies on sequence similarities allowed scientists to understand how CRISPRs work alongside CRISPR-associated enzymes (Cas) as a system (CRISPR-Cas) to protect prokaryotic cells against invading viruses and plasmids. Further studies on CRISPR-Cas systems led to the invention of CRISPR-Cas9 genome editing technology and the 2020 Nobel Prize in Chemistry awarded to Jennifer Doudna and Emanuelle Charpentier.
In the last few years, scientists have harnessed the power of the CRISPR-Cas system as a tool for the specific detection of pathogens with increased sensitivity and portability. In this webinar we will introduce the process for the design, development, and optimization of different CRISPR-Cas methods for the detection of plant pathogens. Speakers will reflect on best practices, lessons learned and future promise for this technology.
View Now
Isothermal Diagnostics for Critical Plant Pathogens: Design, Development, and Implementation
Broadcast date: March 14, 2024 | 1:00pm - 2:30pm CDT
Gain a comprehensive understanding of the fundamental principles of isothermal amplification techniques, explore real-world case studies that showcase successful applications of isothermal diagnostics in plant pathology, and understand how these techniques have been used to identify and manage plant diseases in diverse agricultural contexts.
View Now
Methods and Tools for Plant Pathogen Immunoassay Design, Development, and Analysis
Broadcast Date: February 6, 2024 | 12:00pm - 1:30pm CST
This session will review classical techniques and describe modern techniques for designing, generating, and applying immunoreagents in diagnostic assays, and analyzing data outputs from assays. At the end of this session, attendees will understand the biology and biochemistry behind immunoassays, terminology describing antibodies used in assays and various types of diagnostic immunoassays; novel techniques for discovery and design of antibody targets; and various methods for generating natural and synthetic antibodies. Attendees will learn where to access online APS open-source statistical tools and how to apply them to data outputs from immunoassays in plant pathogen diagnostics.
View Now
Sequence-Based Diagnostic Technology
Broadcast Date: January 24, 2024 | 9:30am - 11:00am CST
This webinar emphasizes use of High Throughput Sequencing (HTS) in pathogen detection and diagnostics, with a focus on sequence data capture, management and pathogen detection. Until recently, HTS has had limited adoption for plant pathogen diagnosis due to the need for specialized sequencing equipment, not designed for diagnostics. Sequence data can be in the Gigabyte size range, making it difficult to manage and organize for pathogen detection. Early protocols require users to have bioinformatics knowledge and ability to code. This webinar will provide solutions to these constraints by pioneers in the field.
View Now
Using RCA for Detecting and Studying Diseases: Examples for Plant DNA Viruses
strong>Broadcast Date: December 6, 2023 | 12:00 - 1:00pm Central
This webinar introduces the protocol for using RCA to amplify circular DNA, focusing on plant DNA viruses as case studies. It begins with a basic DNA extraction protocol and proceeds to the amplification step using phi 29 DNA polymerase. The resulting DNA will then be ready for various applications, including sequencing, cloning, and diversity analysis. At the end of this webinar, attendees will have an understanding of:
- the advantages of employing RCA in molecular biology and diagnostic laboratories
- how to explain the DNA amplification procedure and the potential uses of the resulting DNA
View Now 
