Nucleic Acid Extraction Database​

A comprehensive database compiling a list of Nucleic Acid (NA) extraction methods/techniques used in peer reviewed publications in plant pathogen diagnostic assays since 2011.

The database also includes information on the NA extraction kits, Assay mix kits, and equipment used depending upon the host, matrix and the purpose of the study.


Select the column headers to sort A to Z and/or filter columns by one or more values. Multiple columns can be sorted/filtered at the same time. 

The search box is a single term or an AND based search.


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Assay Mix
Other Kits Used
Equipment Used
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ViewBacteria2021Rathayibacter toxicusSurveillance  PoaceaeGrassRPADNADNeasy Blood & Tissue Kit (Qiagen)
RPA - TwistDx nfo kit (TwistDx Limited, Maidenhead SL6 4XE, UK); PCR - GoTaq Green Master Mix (Promega, Madison, WI)
Plant Material Lysis Kits (OptiGene, West Sussex, UK) - from plant samples
PCR - PTC-200 Peltier thermal cycler and DNA Engine (Bio-Rad, Hercules, CA, USA); RPA - Heat block
Yhttps://doi.org/10.3390/biology10070620
ViewBacteria2020Xanthomonas gardneriScreeningTomato LeafLAMPDNANucleoSpin® Microbial DNA kit (Macherey-Nagel)
LAMP - Isothermal master mix (Optigene, UK); 2x Colorimetric LAMP Master Mix (Cat. No. M1800, New England Biolabs).
NA
RT-LAMP - QuantStudio® 6 Flex Real-Time PCR System (Thermofisher, USA) and BioRangerTM (Diagenetix, USA); Colorimetric LAMP - Heat block
Nhttps://doi.org/10.3390/microorganisms8091301
ViewBacteria2020Xanthomonas gardneriScreeningPepperLeafLAMPDNANucleoSpin® Microbial DNA kit (Macherey-Nagel)
LAMP - Isothermal master mix (Optigene, UK); 2x Colorimetric LAMP Master Mix (Cat. No. M1800, New England Biolabs).
NA
RT-LAMP - QuantStudio® 6 Flex Real-Time PCR System (Thermofisher, USA) and BioRangerTM (Diagenetix, USA); Colorimetric LAMP - Heat block
Nhttps://doi.org/10.3390/microorganisms8091301
ViewBacteria2020Dickeya spp.  DetectionPotatoCulturesReal Time PCRDNA Ultra Clean Microbial DNA Isolation kit (Mo Bio Laboratories)
Multiplex qPCR - Rotor-Gene Multiplex PCR Master Mix (Qiagen)
NA
PCR - Rotor-Gene Q (Qiagen, Germantown, MD); qPCR - Rotor-Gene Q (Qiagen, Germantown, MD)
Nhttps://doi.org/10.1111/jam.14579
ViewBacteria2020Dickeya spp.  DetectionPotatoCulturesReal Time PCRDNAFTA cards
N
ViewBacteria2020Dickeya spp.  DetectionPotatoStemReal Time PCRDNAWizard Genomic DNA Purification Kit (Promega)
Multiplex qPCR - Rotor-Gene Multiplex PCR Master Mix (Qiagen)
NA
PCR - Rotor-Gene Q (Qiagen, Germantown, MD); qPCR - Rotor-Gene Q (Qiagen, Germantown, MD)
Nhttps://doi.org/10.1111/jam.14579
ViewBacteria2020Dickeya spp.  DetectionPotatoTuberReal Time PCRDNAWizard Genomic DNA Purification Kit (Promega)
Multiplex qPCR - Rotor-Gene Multiplex PCR Master Mix (Qiagen)
NA
PCR - Rotor-Gene Q (Qiagen, Germantown, MD); qPCR - Rotor-Gene Q (Qiagen, Germantown, MD)
Nhttps://doi.org/10.1111/jam.14579
ViewBacteria2020Dickeya spp.  DetectionPotatoRootReal Time PCRDNAWizard Genomic DNA Purification Kit (Promega)
Multiplex qPCR - Rotor-Gene Multiplex PCR Master Mix (Qiagen)
NA
PCR - Rotor-Gene Q (Qiagen, Germantown, MD); qPCR - Rotor-Gene Q (Qiagen, Germantown, MD)
Nhttps://doi.org/10.1111/jam.14579
ViewBacteria2020Erwinia amylovoraDetectionAppleStemLAMPDNAQuick DNA extraction from bacterial suspension
qPCR - Itaq universal SYBR green super mix (BioRad Hercules, CA); LAMP reaction mixture: Isothermal enzyme mix (OptiGene)
Immunoassay kits - AgriStrip kit (Bioreba, Reinach, Switzerland) and Pocket Diagnostics kits (Abingdon Health, York, UK)
LAMP - Handheld battery-powered portable device called 'BioRanger' (Diagenetix, Inc., Honolulu, HI) connected to an Android tablet through Bluetooth; qPCR - Bio-Rad CFX96 Touch™ Real-Time PCR Detection System
Nhttps://doi.org/10.1007/s42161-020-00644-w
ViewBacteria2020Erwinia amylovoraDetectionAppleStemReal time PCR DNAQuick DNA extraction from bacterial suspension
qPCR - Itaq universal SYBR green super mix (BioRad Hercules, CA); LAMP reaction mixture: Isothermal enzyme mix (OptiGene)
Immunoassay kits - AgriStrip kit (Bioreba, Reinach, Switzerland) and Pocket Diagnostics kits (Abingdon Health, York, UK)
LAMP - Handheld battery-powered portable device called 'BioRanger' (Diagenetix, Inc., Honolulu, HI) connected to an Android tablet through Bluetooth; qPCR - Bio-Rad CFX96 Touch™ Real-Time PCR Detection System
Nhttps://doi.org/10.1007/s42161-020-00644-w
ViewBacteria2020Xanthomonas fragariaeDetection StrawberryPetiolesReal time PCRDNADNeasy Blood & Tissue kit  (Qiagen)
1X KAPA SYBR FAST qPCR master mix (KAPA Biosystems, Wilmington, MA)
NA
RT-PCR - CFX96 TOUCH Real-Time PCR Detection System (Bio-Rad, Hercules, CA)
Nhttps://doi.org/10.1094/PDIS-10-19-2248-RE
ViewBacteria2020Xanthomonas fragariaeManagement StrawberryPetiolesReal time PCRDNADNeasy Blood & Tissue kit  (Qiagen)
1X KAPA SYBR FAST qPCR master mix (KAPA Biosystems, Wilmington, MA)
NA
RT-PCR - CFX96 TOUCH Real-Time PCR Detection System (Bio-Rad, Hercules, CA)
Nhttps://doi.org/10.1094/PDIS-10-19-2248-RE
ViewBacteria2020Dickeya spp.  DetectionCulturesTubersRPADNAUltraClean Microbial DNA Isolation Kit (Mo Bio Laboratories)
RPA - TwistAmp Exo Kit (TwistDX Limited, Maindenhead, UK)
NA
PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA, U.S.A.); RPA - Used The HybriDetect dipstick
Nhttps://doi.org/10.1094/PDIS-09-19-1988-RE
ViewBacteria2020Dickeya spp.  DetectionPotatoTubersRPADNAWizard Genomic DNA Purification Kit (Promega)
RPA - TwistAmp Exo Kit (TwistDX Limited, Maindenhead, UK)
NA
PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA, U.S.A.); RPA - Used The HybriDetect dipstick
Nhttps://doi.org/10.1094/PDIS-09-19-1988-RE
ViewBacteria2020Dickeya spp.  DetectionTaroTubersRPADNAWizard Genomic DNA Purification Kit (Promega)
RPA - TwistAmp Exo Kit (TwistDX Limited, Maindenhead, UK)
NA
PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA, U.S.A.); RPA - Used The HybriDetect dipstick
Nhttps://doi.org/10.1094/PDIS-09-19-1988-RE
ViewBacteria2020Dickeya spp.  DetectionSweet potatoTubersRPADNAWizard Genomic DNA Purification Kit (Promega)
RPA - TwistAmp Exo Kit (TwistDX Limited, Maindenhead, UK)
NA
PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA, U.S.A.); RPA - Used The HybriDetect dipstick
Nhttps://doi.org/10.1094/PDIS-09-19-1988-RE
ViewBacteria2020Clavibacter michiganensisDetectionTomatoSeedHTSDNACTAB
Master mix not specified, mPCR done usinf BioRad's iProof polymerase,
Wizard Genomic DNA Purification Kit (Promega, Madison, WI)
PCR - C1000 Touch Thermal Cycler (Bio-Rad, Hercules, CA, U.S.A.)
Nhttps://doi.org/10.1094/PHYTO-10-19-0405-R
ViewBacteria2020Clavibacter michiganensisDetectionTomatoSeedMultiplex PCRDNAPhenol-chloroform extraction (Murray and Thompson, 1980)
Master mix not specified, mPCR done usinf BioRad's iProof polymerase,
Wizard Genomic DNA Purification Kit (Promega, Madison, WI)
PCR - C1000 Touch Thermal Cycler (Bio-Rad, Hercules, CA, U.S.A.)
Nhttps://doi.org/10.1094/PHYTO-10-19-0405-R
ViewBacteria2020Xanthomonas vasicola pv. vasculorum DetectionMaizeLeafReal Time PCRDNACTAB
RT-PCR - PrimeTime Gene Expression Master Mix (IDT) with ROX as a passive reference dye; LAMP - Isothermal MasterMix Iso-001 (Optigene, Horsham, UK)
TruSeq Nano DNA Library Prep kit (Illumina, San Diego, CA)
Sequencer - MiSeq platform (Illumina); RT-PCR - QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA) used in Method development lab and QuantStudio 3 Real-Time PCR System (Applied Biosystems) used in the testing lab
Nhttps://doi.org/10.1094/PHYTO-12-18-0453-R
ViewBacteria2020Xanthomonas vasicola pv. vasculorum DiagnosticMaizeSeedLAMPDNABlood & Cell culture DNA Maxi kit (Qiagen)
RT-PCR - PrimeTime Gene Expression Master Mix (IDT) with ROX as a passive reference dye; LAMP - Isothermal MasterMix Iso-001 (Optigene, Horsham, UK)
TruSeq Nano DNA Library Prep kit (Illumina, San Diego, CA)
Sequencer - MiSeq platform (Illumina); RT-PCR - QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA) used in Method development lab and QuantStudio 3 Real-Time PCR System (Applied Biosystems) used in the testing lab
Nhttps://doi.org/10.1094/PHYTO-12-18-0453-R
ViewBacteria2019Xylella fastidiosaDiagnostics BlueberryLeafC-PCRDNADNeasy Plant Mini Kit (Qiagen)
C-PCR:EconoTaq PLUS GREEN 2X Master Mix (Lucigen, Madison, WI); RT-PCR:iQ™ SYBR Green Supermix (BioRad Laboratories Inc., Hercules, CA; LAMP:LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) and for LAMP-PCR amplification used WarmStart1Colorimetric LAMP 2X Master Mix (New England Bio Labs Ltd.,UK)
RPA:AmplifyRP1XRT + for Xf kit (Agdia1Inc. Cat# XCS 34501/0048)
Conventional PCR and real-time PCR - Thermocycler (Biorad-96 well T100™, Bio-Rad, Hercules, CA)
Nhttps://doi.org/10.1371/journal.pone.0221903
ViewBacteria2019Xylella fastidiosaDiagnostics BlueberryLeafReal time PCRDNADNeasy Plant Mini Kit (Qiagen)
C-PCR:EconoTaq PLUS GREEN 2X Master Mix (Lucigen, Madison, WI); RT-PCR:iQ™ SYBR Green Supermix (BioRad Laboratories Inc., Hercules, CA; LAMP:LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) and for LAMP-PCR amplification used WarmStart1Colorimetric LAMP 2X Master Mix (New England Bio Labs Ltd.,UK)
RPA:AmplifyRP1XRT + for Xf kit (Agdia1Inc. Cat# XCS 34501/0048)
Conventional PCR and real-time PCR - Thermocycler (Biorad-96 well T100™, Bio-Rad, Hercules, CA)
Nhttps://doi.org/10.1371/journal.pone.0221903
ViewBacteria2019Xylella fastidiosaDiagnostics BlueberryLeafRPADNADNeasy Plant Mini Kit (Qiagen)
C-PCR:EconoTaq PLUS GREEN 2X Master Mix (Lucigen, Madison, WI); RT-PCR:iQ™ SYBR Green Supermix (BioRad Laboratories Inc., Hercules, CA; LAMP:LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) and for LAMP-PCR amplification used WarmStart1Colorimetric LAMP 2X Master Mix (New England Bio Labs Ltd.,UK)
RPA:AmplifyRP1XRT + for Xf kit (Agdia1Inc. Cat# XCS 34501/0048)
Conventional PCR and real-time PCR - Thermocycler (Biorad-96 well T100™, Bio-Rad, Hercules, CA)
Nhttps://doi.org/10.1371/journal.pone.0221903
ViewBacteria2019Xylella fastidiosaDetectionBlueberryLeafRPADNADNeasy Plant Mini Kit (Qiagen)
C-PCR:EconoTaq PLUS GREEN 2X Master Mix (Lucigen, Madison, WI); RT-PCR:iQ™ SYBR Green Supermix (BioRad Laboratories Inc., Hercules, CA; LAMP:LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) and for LAMP-PCR amplification used WarmStart1Colorimetric LAMP 2X Master Mix (New England Bio Labs Ltd.,UK)
RPA:AmplifyRP1XRT + for Xf kit (Agdia1Inc. Cat# XCS 34501/0048)
Conventional PCR and real-time PCR - Thermocycler (Biorad-96 well T100™, Bio-Rad, Hercules, CA)
Nhttps://doi.org/10.1371/journal.pone.0221903
ViewBacteria2019Xylella fastidiosaDetectionBlueberryLeafRPADNADNeasy Plant Mini Kit (Qiagen)
C-PCR:EconoTaq PLUS GREEN 2X Master Mix (Lucigen, Madison, WI); RT-PCR:iQ™ SYBR Green Supermix (BioRad Laboratories Inc., Hercules, CA; LAMP:LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) and for LAMP-PCR amplification used WarmStart1Colorimetric LAMP 2X Master Mix (New England Bio Labs Ltd.,UK)
RPA:AmplifyRP1XRT + for Xf kit (Agdia1Inc. Cat# XCS 34501/0048)
Conventional PCR and real-time PCR - Thermocycler (Biorad-96 well T100™, Bio-Rad, Hercules, CA)
Nhttps://doi.org/10.1371/journal.pone.0221903
ViewBacteria2019Pantoea alliiDetection OnionsBulbsReverse Transcriptase PCRDNAWizard Genomic DNA Purification Kit (Promega)
TaqMan Real Time PCR - 2× SsoAdvanced Universal Probes Supermix (Bio-Rad)
NA
RT-PCR - Chromo4 real-time PCR system (MJ Research Bio-Rad Laboratories) and Rotor-Gene Q real-time PCR machine (Qiagen)
Nhttps://doi.org/10.1094/PDIS-03-19-0563-RE
ViewBacteria2019Pantoea alliiScreeningOnionsBulbsReverse Transcriptase PCRDNAWizard Genomic DNA Purification Kit (Promega)
TaqMan Real Time PCR - 2× SsoAdvanced Universal Probes Supermix (Bio-Rad)
NA
RT-PCR - Chromo4 real-time PCR system (MJ Research Bio-Rad Laboratories) and Rotor-Gene Q real-time PCR machine (Qiagen)
Nhttps://doi.org/10.1094/PDIS-03-19-0563-RE
ViewBacteria2018Pectobacterium sp.Diagnostics PotatoTubersRPADNAWizard Genomic DNA Purification Kit (Promega)
PCR - GoTaq Green Master Mix (Promega)
TwistAmp Exo® kit for the RPA reactions
PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA)
Nhttps://doi.org/10.1038/s41598-018-34275-0
ViewBacteria2018Pectobacterium sp.Diagnostics TomatoTubersRPADNAWizard Genomic DNA Purification Kit (Promega)
PCR - GoTaq Green Master Mix (Promega)
TwistAmp Exo® kit for the RPA reactions
PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA)
Nhttps://doi.org/10.1038/s41598-018-34275-0
ViewBacteria2018Pectobacterium sp.Diagnostics PepperTubersRPADNAWizard Genomic DNA Purification Kit (Promega)
PCR - GoTaq Green Master Mix (Promega)
TwistAmp Exo® kit for the RPA reactions
PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA)
Nhttps://doi.org/10.1038/s41598-018-34275-0
ViewBacteria2018Pectobacterium sp.Diagnostics TobaccoTubersRPADNAWizard Genomic DNA Purification Kit (Promega)
PCR - GoTaq Green Master Mix (Promega)
TwistAmp Exo® kit for the RPA reactions
PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA)
Nhttps://doi.org/10.1038/s41598-018-34275-0
ViewBacteria2018Pectobacterium sp.Diagnostics BroccoliTubersRPADNAWizard Genomic DNA Purification Kit (Promega)
PCR - GoTaq Green Master Mix (Promega)
TwistAmp Exo® kit for the RPA reactions
PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA)
Nhttps://doi.org/10.1038/s41598-018-34275-0
ViewBacteria2018Pectobacterium sp.Diagnostics TubersTubersRPADNAWizard Genomic DNA Purification Kit (Promega)
PCR - GoTaq Green Master Mix (Promega)
TwistAmp Exo® kit for the RPA reactions
PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA)
Nhttps://doi.org/10.1038/s41598-018-34275-0
ViewBacteria2018Xanthomonas euvesicatoriaDetectionTomato LeafLAMP  DNAWizard Genomic DNA Purification Kit (Promega)
LAMP:Optigene® Master Mix (Optigene, West Sussex, UK)
NA
LAMP - Bio-Ranger™ (Diagenetix Inc, Honolulu, HI); FOTO/UV® 26 transilluminator (Fotodyne Inc., WI) - Used for visualizing LAMP assay results with SYBR Green dye
Nhttps://doi.org/10.1038/s41598-018-32295-4
ViewBacteria2018Xanthomonas euvesicatoriaDetectionTomato LeafLAMP  DNAUltra Clean Microbial DNA Isolation Kit (Mo Bio Laboratories)
LAMP:Optigene® Master Mix (Optigene, West Sussex, UK)
NA
LAMP - Bio-Ranger™ (Diagenetix Inc, Honolulu, HI); FOTO/UV® 26 transilluminator (Fotodyne Inc., WI) - Used for visualizing LAMP assay results with SYBR Green dye
Nhttps://doi.org/10.1038/s41598-018-32295-4
ViewBacteria2016Ralstonia solanacearumDiagnosticsTomatoSeeds Real Time PCRDNADNeasy Blood & Tissue Kit (Qiagen)
Taq qPCR master mix (Invitogen)
NA
TAQMAN qPCR - Smart Cycler (Cepheid, Inc, Sunnyvale, CA) and CFX96 thermo-cycler (Bio-Rad Laboratories, Hercules, CA)
Yhttps://doi.org/10.1007/s00284-016-1091-z
ViewBacteria2016Ralstonia solanacearumDiagnosticsTobaccoSeeds Real Time PCRDNADNeasy Blood & Tissue Kit (Qiagen)
Taq qPCR master mix (Invitogen)
NA
TAQMAN qPCR - Smart Cycler (Cepheid, Inc, Sunnyvale, CA) and CFX96 thermo-cycler (Bio-Rad Laboratories, Hercules, CA)
Yhttps://doi.org/10.1007/s00284-016-1091-z
ViewBacteria2016Ralstonia solanacearumDiagnosticsGeranium Seeds Real Time PCRDNADNeasy Blood & Tissue Kit (Qiagen)
Taq qPCR master mix (Invitogen)
NA
TAQMAN qPCR - Smart Cycler (Cepheid, Inc, Sunnyvale, CA) and CFX96 thermo-cycler (Bio-Rad Laboratories, Hercules, CA)
Yhttps://doi.org/10.1007/s00284-016-1091-z
ViewBacteria2016Ralstonia solanacearumDiagnosticsPotatoSeeds Real Time PCRDNADNeasy Blood & Tissue Kit (Qiagen)
Taq qPCR master mix (Invitogen)
NA
TAQMAN qPCR - Smart Cycler (Cepheid, Inc, Sunnyvale, CA) and CFX96 thermo-cycler (Bio-Rad Laboratories, Hercules, CA)
Yhttps://doi.org/10.1007/s00284-016-1091-z
ViewBacteria2016Candidatus Liberibacter asiaticusDiagnosticsCitrus LeafReal-time PCRDNAE. Z. N. A. HP Plant DNA Kit (OMEGA Bio-Tek)
RT-PCR - iQ™ SYBR® Green Supermix (Bio-Rad) or Fast SYBR® Green Master Mix (Applied Biosystems) or Bestar® qPCR Master Mix (DBI® Bioscience); TaqMan assay - TaqMan® Fast Universal PCR Master Mix (2X) (Applied Biosystems)
NA
Applied Biosystems StepOnePlus™ Real-Time PCR Systems (Used for SYBR Green and TaqMan real-time PCR in the USA); Bio-Rad CFX Connect Real-Time System (Used for SYBR Green real-time PCR in China)
Yhttp://DOI:10.1038/srep39020
ViewBacteria2016Candidatus Liberibacter asiaticusDiagnosticsCitrus LeafReal-time PCRDNADNeasy Plant Mini Kit (Qiagen)
RT-PCR - iQ™ SYBR® Green Supermix (Bio-Rad) or Fast SYBR® Green Master Mix (Applied Biosystems) or Bestar® qPCR Master Mix (DBI® Bioscience); TaqMan assay - TaqMan® Fast Universal PCR Master Mix (2X) (Applied Biosystems)
NA
Applied Biosystems StepOnePlus™ Real-Time PCR Systems (Used for SYBR Green and TaqMan real-time PCR in the USA); Bio-Rad CFX Connect Real-Time System (Used for SYBR Green real-time PCR in China)
Yhttp://DOI:10.1038/srep39020
ViewBacteria2016Candidatus Liberibacter asiaticusDiagnosticsCitrus LeafReal-time PCRDNATIANamp Genomic DNA Kit (Tiangen Biotech)
RT-PCR - iQ™ SYBR® Green Supermix (Bio-Rad) or Fast SYBR® Green Master Mix (Applied Biosystems) or Bestar® qPCR Master Mix (DBI® Bioscience); TaqMan assay - TaqMan® Fast Universal PCR Master Mix (2X) (Applied Biosystems)
NA
Applied Biosystems StepOnePlus™ Real-Time PCR Systems (Used for SYBR Green and TaqMan real-time PCR in the USA); Bio-Rad CFX Connect Real-Time System (Used for SYBR Green real-time PCR in China)
Yhttp://DOI:10.1038/srep39020
ViewBacteria2016Candidatus Liberibacter asiaticusDiagnosticsCitrus LeafReal-time PCRDNADNeasy Blood & Tissue Kit (Qiagen)
RT-PCR - iQ™ SYBR® Green Supermix (Bio-Rad) or Fast SYBR® Green Master Mix (Applied Biosystems) or Bestar® qPCR Master Mix (DBI® Bioscience); TaqMan assay - TaqMan® Fast Universal PCR Master Mix (2X) (Applied Biosystems)
NA
Applied Biosystems StepOnePlus™ Real-Time PCR Systems (Used for SYBR Green and TaqMan real-time PCR in the USA); Bio-Rad CFX Connect Real-Time System (Used for SYBR Green real-time PCR in China)
Yhttp://DOI:10.1038/srep39020
ViewBacteria2016Clavibacter michiganensis subsp. NebraskensisDetection CornLeafMultiplex RT-PCRDNAUltraClean soil DNA isolation kit (Mo Bio Laboratories)
PCR - Titanium Taq DNA polymerase; TaqMan assay - 2X Sso Universal  Probes supermix (BioRad Lab)
NA
Sequencing - ABI 3130xl Automated Sequencer (Applied Biosystems/Hitachi) and Illumina MiSeq Technology; TaqMan RT-PCR - Chromo4 Real-Time PCR System (MJ Research Bio-Rad Laboratories) and LightCycler 480 Instrument (Roche)
Nhttps://doi.org/10.1094/PHYTO-05-16-0188-R
ViewBacteria2016Clavibacter michiganensis subsp. NebraskensisDiagnosticsCornLeafMultiplex RT-PCRDNAWizard Genomic DNA Purification Kit (Promega)
PCR - Titanium Taq DNA polymerase; TaqMan assay - 2X Sso Universal  Probes supermix (BioRad Lab)
NA
Sequencing - ABI 3130xl Automated Sequencer (Applied Biosystems/Hitachi) and Illumina MiSeq Technology; TaqMan RT-PCR - Chromo4 Real-Time PCR System (MJ Research Bio-Rad Laboratories) and LightCycler 480 Instrument (Roche)
Nhttps://doi.org/10.1094/PHYTO-05-16-0188-R
ViewBacteria2015Candidatus Liberibacter asiaticusSurveillance Citrus Plant LAMPDNADNeasy Plant Mini Kit (Qiagen)
qPCR: Qiagen® Magmax kit (Qiagen Inc. CA); ISO-001 master mix (obtained from Optigene® Limited, West Sussex, United Kingdom) for LAMP assays
NA
Smart-DART™ platform from Diagenetix Inc.™ - psyllid DNA extraction and LAMP; qPCR - Stratagene qPCR machine
Yhttp://dx.doi.org/10.1016/
ViewBacteria2015Candidatus Liberibacter asiaticusSurveillance Citrus PsyllidsLAMPDNADNeasy Plant Mini Kit (Qiagen)
qPCR: Qiagen® Magmax kit (Qiagen Inc. CA); ISO-001 master mix (obtained from Optigene® Limited, West Sussex, United Kingdom) for LAMP assays
NA
Smart-DART™ platform from Diagenetix Inc.™ - psyllid DNA extraction and LAMP; qPCR - Stratagene qPCR machine
Yhttp://dx.doi.org/10.1016/
ViewBacteria2013Pseudomonas syringae pv. actinidiaeDiversityKiwifruitLeafPCRDNAPureLink Genomic DNA Mini kit (Invitrogen)
Multiplex PCR - GoTaq Colorless Master Mix 2X (Promega Corporation)
NA
PCR - C1000 gradient thermal cycler (Bio-Rad Laboratories srl)
Nhttp://dx.doi.org/10.1094/ PDIS-06-12-0590-RE
ViewBacteria2013Pseudomonas syringae pv. actinidiaeDiversityKiwifruitLeafPCRDNADNeasy Plant Mini Kit (Qiagen)
Multiplex PCR - GoTaq Colorless Master Mix 2X (Promega Corporation)
NA
PCR - C1000 gradient thermal cycler (Bio-Rad Laboratories srl)
Nhttp://dx.doi.org/10.1094/ PDIS-06-12-0590-RE
ViewFungi2021Colletotrichum acutatumDiagnosticsStrawberryMycelia  HRM assayDNAFastDNA™ SPIN kit  (MP Bio)
PCR - AccuStart II PCR ToughMix (QuantaBio, Beverly, MA)
NA
PCR and HRM analysis - Roche LightCycler 480 Instrument II (Roche Diagnostics, Indianapolis, IN)
Nhttps://doi.org/10.1094/PHYTO-12-20-0556-R
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