| View | Bacteria | 2021 | Rathayibacter toxicus | Surveillance | Poaceae | Grass | RPA | DNA | DNeasy Blood & Tissue Kit (Qiagen) | RPA - TwistDx nfo kit (TwistDx Limited, Maidenhead SL6 4XE, UK); PCR - GoTaq Green Master Mix (Promega, Madison, WI) | Plant Material Lysis Kits (OptiGene, West Sussex, UK) - from plant samples | PCR - PTC-200 Peltier thermal cycler and DNA Engine (Bio-Rad, Hercules, CA, USA); RPA - Heat block | Y | https://doi.org/10.3390/biology10070620 |
| View | Bacteria | 2020 | Xanthomonas gardneri | Screening | Tomato | Leaf | LAMP | DNA | NucleoSpin® Microbial DNA kit (Macherey-Nagel) | LAMP - Isothermal master mix (Optigene, UK); 2x Colorimetric LAMP Master Mix (Cat. No. M1800, New England Biolabs). | NA | RT-LAMP - QuantStudio® 6 Flex Real-Time PCR System (Thermofisher, USA) and BioRangerTM (Diagenetix, USA); Colorimetric LAMP - Heat block | N | https://doi.org/10.3390/microorganisms8091301 |
| View | Bacteria | 2020 | Xanthomonas gardneri | Screening | Pepper | Leaf | LAMP | DNA | NucleoSpin® Microbial DNA kit (Macherey-Nagel) | LAMP - Isothermal master mix (Optigene, UK); 2x Colorimetric LAMP Master Mix (Cat. No. M1800, New England Biolabs). | NA | RT-LAMP - QuantStudio® 6 Flex Real-Time PCR System (Thermofisher, USA) and BioRangerTM (Diagenetix, USA); Colorimetric LAMP - Heat block | N | https://doi.org/10.3390/microorganisms8091301 |
| View | Bacteria | 2020 | Dickeya spp. | Detection | Potato | Cultures | Real Time PCR | DNA | Ultra Clean Microbial DNA Isolation kit (Mo Bio Laboratories) | Multiplex qPCR - Rotor-Gene Multiplex PCR Master Mix (Qiagen) | NA | PCR - Rotor-Gene Q (Qiagen, Germantown, MD); qPCR - Rotor-Gene Q (Qiagen, Germantown, MD) | N | https://doi.org/10.1111/jam.14579 |
| View | Bacteria | 2020 | Dickeya spp. | Detection | Potato | Cultures | Real Time PCR | DNA | FTA cards | | | | N | |
| View | Bacteria | 2020 | Dickeya spp. | Detection | Potato | Stem | Real Time PCR | DNA | Wizard Genomic DNA Purification Kit (Promega) | Multiplex qPCR - Rotor-Gene Multiplex PCR Master Mix (Qiagen) | NA | PCR - Rotor-Gene Q (Qiagen, Germantown, MD); qPCR - Rotor-Gene Q (Qiagen, Germantown, MD) | N | https://doi.org/10.1111/jam.14579 |
| View | Bacteria | 2020 | Dickeya spp. | Detection | Potato | Tuber | Real Time PCR | DNA | Wizard Genomic DNA Purification Kit (Promega) | Multiplex qPCR - Rotor-Gene Multiplex PCR Master Mix (Qiagen) | NA | PCR - Rotor-Gene Q (Qiagen, Germantown, MD); qPCR - Rotor-Gene Q (Qiagen, Germantown, MD) | N | https://doi.org/10.1111/jam.14579 |
| View | Bacteria | 2020 | Dickeya spp. | Detection | Potato | Root | Real Time PCR | DNA | Wizard Genomic DNA Purification Kit (Promega) | Multiplex qPCR - Rotor-Gene Multiplex PCR Master Mix (Qiagen) | NA | PCR - Rotor-Gene Q (Qiagen, Germantown, MD); qPCR - Rotor-Gene Q (Qiagen, Germantown, MD) | N | https://doi.org/10.1111/jam.14579 |
| View | Bacteria | 2020 | Erwinia amylovora | Detection | Apple | Stem | LAMP | DNA | Quick DNA extraction from bacterial suspension | qPCR - Itaq universal SYBR green super mix (BioRad Hercules, CA); LAMP reaction mixture: Isothermal enzyme mix (OptiGene) | Immunoassay kits - AgriStrip kit (Bioreba, Reinach, Switzerland) and Pocket Diagnostics kits (Abingdon Health, York, UK) | LAMP - Handheld battery-powered portable device called 'BioRanger' (Diagenetix, Inc., Honolulu, HI) connected to an Android tablet through Bluetooth; qPCR - Bio-Rad CFX96 Touch™ Real-Time PCR Detection System | N | https://doi.org/10.1007/s42161-020-00644-w |
| View | Bacteria | 2020 | Erwinia amylovora | Detection | Apple | Stem | Real time PCR | DNA | Quick DNA extraction from bacterial suspension | qPCR - Itaq universal SYBR green super mix (BioRad Hercules, CA); LAMP reaction mixture: Isothermal enzyme mix (OptiGene) | Immunoassay kits - AgriStrip kit (Bioreba, Reinach, Switzerland) and Pocket Diagnostics kits (Abingdon Health, York, UK) | LAMP - Handheld battery-powered portable device called 'BioRanger' (Diagenetix, Inc., Honolulu, HI) connected to an Android tablet through Bluetooth; qPCR - Bio-Rad CFX96 Touch™ Real-Time PCR Detection System | N | https://doi.org/10.1007/s42161-020-00644-w |
| View | Bacteria | 2020 | Xanthomonas fragariae | Detection | Strawberry | Petioles | Real time PCR | DNA | DNeasy Blood & Tissue kit (Qiagen) | 1X KAPA SYBR FAST qPCR master mix (KAPA Biosystems, Wilmington, MA) | NA | RT-PCR - CFX96 TOUCH Real-Time PCR Detection System (Bio-Rad, Hercules, CA) | N | https://doi.org/10.1094/PDIS-10-19-2248-RE |
| View | Bacteria | 2020 | Xanthomonas fragariae | Management | Strawberry | Petioles | Real time PCR | DNA | DNeasy Blood & Tissue kit (Qiagen) | 1X KAPA SYBR FAST qPCR master mix (KAPA Biosystems, Wilmington, MA) | NA | RT-PCR - CFX96 TOUCH Real-Time PCR Detection System (Bio-Rad, Hercules, CA) | N | https://doi.org/10.1094/PDIS-10-19-2248-RE |
| View | Bacteria | 2020 | Dickeya spp. | Detection | Cultures | Tubers | RPA | DNA | UltraClean Microbial DNA Isolation Kit (Mo Bio Laboratories) | RPA - TwistAmp Exo Kit (TwistDX Limited, Maindenhead, UK) | NA | PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA, U.S.A.); RPA - Used The HybriDetect dipstick | N | https://doi.org/10.1094/PDIS-09-19-1988-RE |
| View | Bacteria | 2020 | Dickeya spp. | Detection | Potato | Tubers | RPA | DNA | Wizard Genomic DNA Purification Kit (Promega) | RPA - TwistAmp Exo Kit (TwistDX Limited, Maindenhead, UK) | NA | PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA, U.S.A.); RPA - Used The HybriDetect dipstick | N | https://doi.org/10.1094/PDIS-09-19-1988-RE |
| View | Bacteria | 2020 | Dickeya spp. | Detection | Taro | Tubers | RPA | DNA | Wizard Genomic DNA Purification Kit (Promega) | RPA - TwistAmp Exo Kit (TwistDX Limited, Maindenhead, UK) | NA | PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA, U.S.A.); RPA - Used The HybriDetect dipstick | N | https://doi.org/10.1094/PDIS-09-19-1988-RE |
| View | Bacteria | 2020 | Dickeya spp. | Detection | Sweet potato | Tubers | RPA | DNA | Wizard Genomic DNA Purification Kit (Promega) | RPA - TwistAmp Exo Kit (TwistDX Limited, Maindenhead, UK) | NA | PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA, U.S.A.); RPA - Used The HybriDetect dipstick | N | https://doi.org/10.1094/PDIS-09-19-1988-RE |
| View | Bacteria | 2020 | Clavibacter michiganensis | Detection | Tomato | Seed | HTS | DNA | CTAB | Master mix not specified, mPCR done usinf BioRad's iProof polymerase, | Wizard Genomic DNA Purification Kit (Promega, Madison, WI) | PCR - C1000 Touch Thermal Cycler (Bio-Rad, Hercules, CA, U.S.A.) | N | https://doi.org/10.1094/PHYTO-10-19-0405-R |
| View | Bacteria | 2020 | Clavibacter michiganensis | Detection | Tomato | Seed | Multiplex PCR | DNA | Phenol-chloroform extraction (Murray and Thompson, 1980) | Master mix not specified, mPCR done usinf BioRad's iProof polymerase, | Wizard Genomic DNA Purification Kit (Promega, Madison, WI) | PCR - C1000 Touch Thermal Cycler (Bio-Rad, Hercules, CA, U.S.A.) | N | https://doi.org/10.1094/PHYTO-10-19-0405-R |
| View | Bacteria | 2020 | Xanthomonas vasicola pv. vasculorum | Detection | Maize | Leaf | Real Time PCR | DNA | CTAB | RT-PCR - PrimeTime Gene Expression Master Mix (IDT) with ROX as a passive reference dye; LAMP - Isothermal MasterMix Iso-001 (Optigene, Horsham, UK) | TruSeq Nano DNA Library Prep kit (Illumina, San Diego, CA) | Sequencer - MiSeq platform (Illumina); RT-PCR - QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA) used in Method development lab and QuantStudio 3 Real-Time PCR System (Applied Biosystems) used in the testing lab | N | https://doi.org/10.1094/PHYTO-12-18-0453-R |
| View | Bacteria | 2020 | Xanthomonas vasicola pv. vasculorum | Diagnostic | Maize | Seed | LAMP | DNA | Blood & Cell culture DNA Maxi kit (Qiagen) | RT-PCR - PrimeTime Gene Expression Master Mix (IDT) with ROX as a passive reference dye; LAMP - Isothermal MasterMix Iso-001 (Optigene, Horsham, UK) | TruSeq Nano DNA Library Prep kit (Illumina, San Diego, CA) | Sequencer - MiSeq platform (Illumina); RT-PCR - QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA) used in Method development lab and QuantStudio 3 Real-Time PCR System (Applied Biosystems) used in the testing lab | N | https://doi.org/10.1094/PHYTO-12-18-0453-R |
| View | Bacteria | 2019 | Xylella fastidiosa | Diagnostics | Blueberry | Leaf | C-PCR | DNA | DNeasy Plant Mini Kit (Qiagen) | C-PCR:EconoTaq PLUS GREEN 2X Master Mix (Lucigen, Madison, WI); RT-PCR:iQ™ SYBR Green Supermix (BioRad Laboratories Inc., Hercules, CA; LAMP:LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) and for LAMP-PCR amplification used WarmStart1Colorimetric LAMP 2X Master Mix (New England Bio Labs Ltd.,UK) | RPA:AmplifyRP1XRT + for Xf kit (Agdia1Inc. Cat# XCS 34501/0048) | Conventional PCR and real-time PCR - Thermocycler (Biorad-96 well T100™, Bio-Rad, Hercules, CA) | N | https://doi.org/10.1371/journal.pone.0221903 |
| View | Bacteria | 2019 | Xylella fastidiosa | Diagnostics | Blueberry | Leaf | Real time PCR | DNA | DNeasy Plant Mini Kit (Qiagen) | C-PCR:EconoTaq PLUS GREEN 2X Master Mix (Lucigen, Madison, WI); RT-PCR:iQ™ SYBR Green Supermix (BioRad Laboratories Inc., Hercules, CA; LAMP:LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) and for LAMP-PCR amplification used WarmStart1Colorimetric LAMP 2X Master Mix (New England Bio Labs Ltd.,UK) | RPA:AmplifyRP1XRT + for Xf kit (Agdia1Inc. Cat# XCS 34501/0048) | Conventional PCR and real-time PCR - Thermocycler (Biorad-96 well T100™, Bio-Rad, Hercules, CA) | N | https://doi.org/10.1371/journal.pone.0221903 |
| View | Bacteria | 2019 | Xylella fastidiosa | Diagnostics | Blueberry | Leaf | RPA | DNA | DNeasy Plant Mini Kit (Qiagen) | C-PCR:EconoTaq PLUS GREEN 2X Master Mix (Lucigen, Madison, WI); RT-PCR:iQ™ SYBR Green Supermix (BioRad Laboratories Inc., Hercules, CA; LAMP:LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) and for LAMP-PCR amplification used WarmStart1Colorimetric LAMP 2X Master Mix (New England Bio Labs Ltd.,UK) | RPA:AmplifyRP1XRT + for Xf kit (Agdia1Inc. Cat# XCS 34501/0048) | Conventional PCR and real-time PCR - Thermocycler (Biorad-96 well T100™, Bio-Rad, Hercules, CA) | N | https://doi.org/10.1371/journal.pone.0221903 |
| View | Bacteria | 2019 | Xylella fastidiosa | Detection | Blueberry | Leaf | RPA | DNA | DNeasy Plant Mini Kit (Qiagen) | C-PCR:EconoTaq PLUS GREEN 2X Master Mix (Lucigen, Madison, WI); RT-PCR:iQ™ SYBR Green Supermix (BioRad Laboratories Inc., Hercules, CA; LAMP:LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) and for LAMP-PCR amplification used WarmStart1Colorimetric LAMP 2X Master Mix (New England Bio Labs Ltd.,UK) | RPA:AmplifyRP1XRT + for Xf kit (Agdia1Inc. Cat# XCS 34501/0048) | Conventional PCR and real-time PCR - Thermocycler (Biorad-96 well T100™, Bio-Rad, Hercules, CA) | N | https://doi.org/10.1371/journal.pone.0221903 |
| View | Bacteria | 2019 | Xylella fastidiosa | Detection | Blueberry | Leaf | RPA | DNA | DNeasy Plant Mini Kit (Qiagen) | C-PCR:EconoTaq PLUS GREEN 2X Master Mix (Lucigen, Madison, WI); RT-PCR:iQ™ SYBR Green Supermix (BioRad Laboratories Inc., Hercules, CA; LAMP:LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) and for LAMP-PCR amplification used WarmStart1Colorimetric LAMP 2X Master Mix (New England Bio Labs Ltd.,UK) | RPA:AmplifyRP1XRT + for Xf kit (Agdia1Inc. Cat# XCS 34501/0048) | Conventional PCR and real-time PCR - Thermocycler (Biorad-96 well T100™, Bio-Rad, Hercules, CA) | N | https://doi.org/10.1371/journal.pone.0221903 |
| View | Bacteria | 2019 | Pantoea allii | Detection | Onions | Bulbs | Reverse Transcriptase PCR | DNA | Wizard Genomic DNA Purification Kit (Promega) | TaqMan Real Time PCR - 2× SsoAdvanced Universal Probes Supermix (Bio-Rad) | NA | RT-PCR - Chromo4 real-time PCR system (MJ Research Bio-Rad Laboratories) and Rotor-Gene Q real-time PCR machine (Qiagen) | N | https://doi.org/10.1094/PDIS-03-19-0563-RE |
| View | Bacteria | 2019 | Pantoea allii | Screening | Onions | Bulbs | Reverse Transcriptase PCR | DNA | Wizard Genomic DNA Purification Kit (Promega) | TaqMan Real Time PCR - 2× SsoAdvanced Universal Probes Supermix (Bio-Rad) | NA | RT-PCR - Chromo4 real-time PCR system (MJ Research Bio-Rad Laboratories) and Rotor-Gene Q real-time PCR machine (Qiagen) | N | https://doi.org/10.1094/PDIS-03-19-0563-RE |
| View | Bacteria | 2018 | Pectobacterium sp. | Diagnostics | Potato | Tubers | RPA | DNA | Wizard Genomic DNA Purification Kit (Promega) | PCR - GoTaq Green Master Mix (Promega) | TwistAmp Exo® kit for the RPA reactions | PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA) | N | https://doi.org/10.1038/s41598-018-34275-0 |
| View | Bacteria | 2018 | Pectobacterium sp. | Diagnostics | Tomato | Tubers | RPA | DNA | Wizard Genomic DNA Purification Kit (Promega) | PCR - GoTaq Green Master Mix (Promega) | TwistAmp Exo® kit for the RPA reactions | PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA) | N | https://doi.org/10.1038/s41598-018-34275-0 |
| View | Bacteria | 2018 | Pectobacterium sp. | Diagnostics | Pepper | Tubers | RPA | DNA | Wizard Genomic DNA Purification Kit (Promega) | PCR - GoTaq Green Master Mix (Promega) | TwistAmp Exo® kit for the RPA reactions | PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA) | N | https://doi.org/10.1038/s41598-018-34275-0 |
| View | Bacteria | 2018 | Pectobacterium sp. | Diagnostics | Tobacco | Tubers | RPA | DNA | Wizard Genomic DNA Purification Kit (Promega) | PCR - GoTaq Green Master Mix (Promega) | TwistAmp Exo® kit for the RPA reactions | PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA) | N | https://doi.org/10.1038/s41598-018-34275-0 |
| View | Bacteria | 2018 | Pectobacterium sp. | Diagnostics | Broccoli | Tubers | RPA | DNA | Wizard Genomic DNA Purification Kit (Promega) | PCR - GoTaq Green Master Mix (Promega) | TwistAmp Exo® kit for the RPA reactions | PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA) | N | https://doi.org/10.1038/s41598-018-34275-0 |
| View | Bacteria | 2018 | Pectobacterium sp. | Diagnostics | Tubers | Tubers | RPA | DNA | Wizard Genomic DNA Purification Kit (Promega) | PCR - GoTaq Green Master Mix (Promega) | TwistAmp Exo® kit for the RPA reactions | PCR - T100 Thermal cycler (Bio-Rad, Hercules, CA) | N | https://doi.org/10.1038/s41598-018-34275-0 |
| View | Bacteria | 2018 | Xanthomonas euvesicatoria | Detection | Tomato | Leaf | LAMP | DNA | Wizard Genomic DNA Purification Kit (Promega) | LAMP:Optigene® Master Mix (Optigene, West Sussex, UK) | NA | LAMP - Bio-Ranger™ (Diagenetix Inc, Honolulu, HI); FOTO/UV® 26 transilluminator (Fotodyne Inc., WI) - Used for visualizing LAMP assay results with SYBR Green dye | N | https://doi.org/10.1038/s41598-018-32295-4 |
| View | Bacteria | 2018 | Xanthomonas euvesicatoria | Detection | Tomato | Leaf | LAMP | DNA | Ultra Clean Microbial DNA Isolation Kit (Mo Bio Laboratories) | LAMP:Optigene® Master Mix (Optigene, West Sussex, UK) | NA | LAMP - Bio-Ranger™ (Diagenetix Inc, Honolulu, HI); FOTO/UV® 26 transilluminator (Fotodyne Inc., WI) - Used for visualizing LAMP assay results with SYBR Green dye | N | https://doi.org/10.1038/s41598-018-32295-4 |
| View | Bacteria | 2016 | Ralstonia solanacearum | Diagnostics | Tomato | Seeds | Real Time PCR | DNA | DNeasy Blood & Tissue Kit (Qiagen) | Taq qPCR master mix (Invitogen) | NA | TAQMAN qPCR - Smart Cycler (Cepheid, Inc, Sunnyvale, CA) and CFX96 thermo-cycler (Bio-Rad Laboratories, Hercules, CA) | Y | https://doi.org/10.1007/s00284-016-1091-z |
| View | Bacteria | 2016 | Ralstonia solanacearum | Diagnostics | Tobacco | Seeds | Real Time PCR | DNA | DNeasy Blood & Tissue Kit (Qiagen) | Taq qPCR master mix (Invitogen) | NA | TAQMAN qPCR - Smart Cycler (Cepheid, Inc, Sunnyvale, CA) and CFX96 thermo-cycler (Bio-Rad Laboratories, Hercules, CA) | Y | https://doi.org/10.1007/s00284-016-1091-z |
| View | Bacteria | 2016 | Ralstonia solanacearum | Diagnostics | Geranium | Seeds | Real Time PCR | DNA | DNeasy Blood & Tissue Kit (Qiagen) | Taq qPCR master mix (Invitogen) | NA | TAQMAN qPCR - Smart Cycler (Cepheid, Inc, Sunnyvale, CA) and CFX96 thermo-cycler (Bio-Rad Laboratories, Hercules, CA) | Y | https://doi.org/10.1007/s00284-016-1091-z |
| View | Bacteria | 2016 | Ralstonia solanacearum | Diagnostics | Potato | Seeds | Real Time PCR | DNA | DNeasy Blood & Tissue Kit (Qiagen) | Taq qPCR master mix (Invitogen) | NA | TAQMAN qPCR - Smart Cycler (Cepheid, Inc, Sunnyvale, CA) and CFX96 thermo-cycler (Bio-Rad Laboratories, Hercules, CA) | Y | https://doi.org/10.1007/s00284-016-1091-z |
| View | Bacteria | 2016 | Candidatus Liberibacter asiaticus | Diagnostics | Citrus | Leaf | Real-time PCR | DNA | E. Z. N. A. HP Plant DNA Kit (OMEGA Bio-Tek) | RT-PCR - iQ™ SYBR® Green Supermix (Bio-Rad) or Fast SYBR® Green Master Mix (Applied Biosystems) or Bestar® qPCR Master Mix (DBI® Bioscience); TaqMan assay - TaqMan® Fast Universal PCR Master Mix (2X) (Applied Biosystems) | NA | Applied Biosystems StepOnePlus™ Real-Time PCR Systems (Used for SYBR Green and TaqMan real-time PCR in the USA); Bio-Rad CFX Connect Real-Time System (Used for SYBR Green real-time PCR in China) | Y | http://DOI:10.1038/srep39020 |
| View | Bacteria | 2016 | Candidatus Liberibacter asiaticus | Diagnostics | Citrus | Leaf | Real-time PCR | DNA | DNeasy Plant Mini Kit (Qiagen) | RT-PCR - iQ™ SYBR® Green Supermix (Bio-Rad) or Fast SYBR® Green Master Mix (Applied Biosystems) or Bestar® qPCR Master Mix (DBI® Bioscience); TaqMan assay - TaqMan® Fast Universal PCR Master Mix (2X) (Applied Biosystems) | NA | Applied Biosystems StepOnePlus™ Real-Time PCR Systems (Used for SYBR Green and TaqMan real-time PCR in the USA); Bio-Rad CFX Connect Real-Time System (Used for SYBR Green real-time PCR in China) | Y | http://DOI:10.1038/srep39020 |
| View | Bacteria | 2016 | Candidatus Liberibacter asiaticus | Diagnostics | Citrus | Leaf | Real-time PCR | DNA | TIANamp Genomic DNA Kit (Tiangen Biotech) | RT-PCR - iQ™ SYBR® Green Supermix (Bio-Rad) or Fast SYBR® Green Master Mix (Applied Biosystems) or Bestar® qPCR Master Mix (DBI® Bioscience); TaqMan assay - TaqMan® Fast Universal PCR Master Mix (2X) (Applied Biosystems) | NA | Applied Biosystems StepOnePlus™ Real-Time PCR Systems (Used for SYBR Green and TaqMan real-time PCR in the USA); Bio-Rad CFX Connect Real-Time System (Used for SYBR Green real-time PCR in China) | Y | http://DOI:10.1038/srep39020 |
| View | Bacteria | 2016 | Candidatus Liberibacter asiaticus | Diagnostics | Citrus | Leaf | Real-time PCR | DNA | DNeasy Blood & Tissue Kit (Qiagen) | RT-PCR - iQ™ SYBR® Green Supermix (Bio-Rad) or Fast SYBR® Green Master Mix (Applied Biosystems) or Bestar® qPCR Master Mix (DBI® Bioscience); TaqMan assay - TaqMan® Fast Universal PCR Master Mix (2X) (Applied Biosystems) | NA | Applied Biosystems StepOnePlus™ Real-Time PCR Systems (Used for SYBR Green and TaqMan real-time PCR in the USA); Bio-Rad CFX Connect Real-Time System (Used for SYBR Green real-time PCR in China) | Y | http://DOI:10.1038/srep39020 |
| View | Bacteria | 2016 | Clavibacter michiganensis subsp. Nebraskensis | Detection | Corn | Leaf | Multiplex RT-PCR | DNA | UltraClean soil DNA isolation kit (Mo Bio Laboratories) | PCR - Titanium Taq DNA polymerase; TaqMan assay - 2X Sso Universal Probes supermix (BioRad Lab) | NA | Sequencing - ABI 3130xl Automated Sequencer (Applied Biosystems/Hitachi) and Illumina MiSeq Technology; TaqMan RT-PCR - Chromo4 Real-Time PCR System (MJ Research Bio-Rad Laboratories) and LightCycler 480 Instrument (Roche) | N | https://doi.org/10.1094/PHYTO-05-16-0188-R |
| View | Bacteria | 2016 | Clavibacter michiganensis subsp. Nebraskensis | Diagnostics | Corn | Leaf | Multiplex RT-PCR | DNA | Wizard Genomic DNA Purification Kit (Promega) | PCR - Titanium Taq DNA polymerase; TaqMan assay - 2X Sso Universal Probes supermix (BioRad Lab) | NA | Sequencing - ABI 3130xl Automated Sequencer (Applied Biosystems/Hitachi) and Illumina MiSeq Technology; TaqMan RT-PCR - Chromo4 Real-Time PCR System (MJ Research Bio-Rad Laboratories) and LightCycler 480 Instrument (Roche) | N | https://doi.org/10.1094/PHYTO-05-16-0188-R |
| View | Bacteria | 2015 | Candidatus Liberibacter asiaticus | Surveillance | Citrus | Plant | LAMP | DNA | DNeasy Plant Mini Kit (Qiagen) | qPCR: Qiagen® Magmax kit (Qiagen Inc. CA); ISO-001 master mix (obtained from Optigene® Limited, West Sussex, United Kingdom) for LAMP assays | NA | Smart-DART™ platform from Diagenetix Inc.™ - psyllid DNA extraction and LAMP; qPCR - Stratagene qPCR machine | Y | http://dx.doi.org/10.1016/ |
| View | Bacteria | 2015 | Candidatus Liberibacter asiaticus | Surveillance | Citrus | Psyllids | LAMP | DNA | DNeasy Plant Mini Kit (Qiagen) | qPCR: Qiagen® Magmax kit (Qiagen Inc. CA); ISO-001 master mix (obtained from Optigene® Limited, West Sussex, United Kingdom) for LAMP assays | NA | Smart-DART™ platform from Diagenetix Inc.™ - psyllid DNA extraction and LAMP; qPCR - Stratagene qPCR machine | Y | http://dx.doi.org/10.1016/ |
| View | Bacteria | 2013 | Pseudomonas syringae pv. actinidiae | Diversity | Kiwifruit | Leaf | PCR | DNA | PureLink Genomic DNA Mini kit (Invitrogen) | Multiplex PCR - GoTaq Colorless Master Mix 2X (Promega Corporation) | NA | PCR - C1000 gradient thermal cycler (Bio-Rad Laboratories srl) | N | http://dx.doi.org/10.1094/ PDIS-06-12-0590-RE |
| View | Bacteria | 2013 | Pseudomonas syringae pv. actinidiae | Diversity | Kiwifruit | Leaf | PCR | DNA | DNeasy Plant Mini Kit (Qiagen) | Multiplex PCR - GoTaq Colorless Master Mix 2X (Promega Corporation) | NA | PCR - C1000 gradient thermal cycler (Bio-Rad Laboratories srl) | N | http://dx.doi.org/10.1094/ PDIS-06-12-0590-RE |
| View | Fungi | 2021 | Colletotrichum acutatum | Diagnostics | Strawberry | Mycelia | HRM assay | DNA | FastDNA™ SPIN kit (MP Bio) | PCR - AccuStart II PCR ToughMix (QuantaBio, Beverly, MA) | NA | PCR and HRM analysis - Roche LightCycler 480 Instrument II (Roche Diagnostics, Indianapolis, IN) | N | https://doi.org/10.1094/PHYTO-12-20-0556-R |