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Wheat yellow mosaic virus Widely Occurring in Wheat (Triticum aestivum) in China

June 2000 , Volume 84 , Number  6
Pages  627 - 630

Chenggui Han , Dawei Li , Yiming Xing , Kun Zhu , Zhaofeng Tian , Zhunan Cai , Jialin Yu , and Yi Liu , National Laboratories for Agrobiotechnology, China Agricultural University, Beijing, China 100094



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Accepted for publication 2 February 2000.
ABSTRACT

Wheat yellow mosaic virus (WYMV) was identified in 25 of 27 wheat (Triticum aestivum) samples from six provinces in China using reverse-transcription polymerase chain reaction. Wheat spindle streak mosaic virus was not detected. Thus, contrary to previous reports, WYMV was the only bymovirus detected in wheat in China. The cDNA fragments corresponding to coat protein coding regions of five WYMV isolates from China were cloned and sequenced. Deduced amino acid comparison of coat proteins among these five isolates, another Chinese WYMV isolate HC, and one isolate from Japan showed very high sequence conservation, particularly in both the N- and C- termini. However, a highly virulent isolate HC from Henan Province showed four amino acid substitutions in highly conserved amino acid positions which might play a role in its pathogenic variation.


Additional keyword: immunosorbent electron microscopy

© 2000 The American Phytopathological Society