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Erigeron Witches'-Broom Phytoplasma in Brazil Represents New Subgroup VII-B in 16S rRNA Gene Group VII, the Ash Yellows Phytoplasma Group

October 2002 , Volume 86 , Number  10
Pages  1,142 - 1,148

Thereza S. L. Barros , USDA-ARS, Molecular Plant Pathology Laboratory, Beltsville, MD 20705, USA, and Laboratório de Virologia e Microscopia Eletrônica, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, 70919-970, Brazil ; Robert E. Davis , USDA-ARS, Molecular Plant Pathology Laboratory, Beltsville, MD ; Renato O. Resende , Laboratorio de Virologia e Microscopia Eletrônica, Departamento de Biologia Celular, Universidade de Brasília, Brasília, DF, 70919-970, Brazil ; and Ellen L. Dally , USDA-ARS, Molecular Plant Pathology Laboratory, Beltsville, MD



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Accepted for publication 23 May 2002.
ABSTRACT

A previously undescribed phytoplasma, Erigeron witches'-broom phytoplasma, was detected in diseased plants of Erigeron sp. and Catharanthus roseus exhibiting symptoms of witches'-broom and chlorosis in the state of São Paulo, Brazil. On the basis of restriction fragment length polymorphism (RFLP) analysis of 16S rDNA amplified in the polymerase chain reaction (PCR), Erigeron witches'-broom phytoplasma was classified in group 16SrVII (ash yellows phytoplasma group), new subgroup VII-B. Phylogenetic analysis of 16S rDNA sequences indicated that this phytoplasma represents a new lineage that is distinct from that of described strains of ash yellows phytoplasma. Erigeron witches'-broom phytoplasma is the first member of the ash yellows phytoplasma group to be recorded in Brazil.


Additional keywords: MLO, mycoplasmalike organism, phylogeny

The American Phytopathological Society, 2002