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A Detached Cucumber Fruit Method to Screen for Resistance to Phytophthora capsici and Effect of Fruit Age on Susceptibility to Infection

Identification and utilization of resistance to Phytophthora capsici could provide the basis for a viable management strategy against cucumber fruit rot, a persistent threat in cucumber (Cucumis sativus) production. Our objectives were to develop a method for testing detached, nonwounded, cucumber fruit for resistance to P. capsici, and to screen cucumber cultivars and plant introductions (collectively referred to as cultigens) for resistance. Four P. capsici isolates (differing in their sensitivity to the fungicide mefenoxam and compatibility type) were compared for their fruit infection capability in 1999 and 2000. No significant differences were found among isolates, and a single isolate was used for all subsequent screens. From 1999 to 2004, 480 cucumber cultigens were grown according to standard practices at Michigan State University research farms in four fields with no history of P. capsici. Commercially mature fruit were harvested, inoculated with P. capsici, and rated for lesion diameter, pathogen sporulation diameter, and density of pathogen sporulation. Although no fruit exhibited complete resistance to P. capsici, some cultigens exhibited limited pathogen sporulation. In the process of screening, it was observed that younger, smaller fruit were comparatively more susceptible than older, larger fruit. Replicated trials with hand-pollinated fruit showed that the transition from susceptible to more resistant appeared to coincide with the transition from the period of rapid fruit elongation to the period of increased fruit diameter. This is the first report using a nonwounded fruit screen to analyze cucumber resistance to P. capsici.