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Genetic Relationships Between Cercospora kikuchii Populations from South America and Japan

First and seventh authors: National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan; second and third authors: Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305-8686, Japan; fourth author: INTA EEA Marcos Juárez, Marcos Juárez, Córdoba 2580, Argentina; fifth and sixth authors: Embrapa Soja, Londrina, Paraná 86001-970, Brazil; and eighth author: National Agricultural Research Center for Tohoku Region, Daisen, Akita 014-0102, Japan

A collection 160 isolates of Cercospora kikuchii was made from South America and 245 from Japan. DNA fingerprint patterns were analyzed based on amplified fragment length polymorphism among the sample isolates, dividing the isolates into seven lineages (I to VII). Partial nucleotide sequence analyses of the β-tubulin gene supported this division into seven lineages. Lineages I and III commonly existed in South America and Japan. In all, 136 of the 160 isolates from South America and 223 of the 245 isolates from Japan belonged to lineage I, indicating that lineage I was the major lineage in each area; 5 isolates from South America and 8 isolates from Japan belonged to lineage III. Lineages II (12 isolates) and IV (2 isolates) were specific to Japan and lineages V (3 isolates), VI (1 isolate), and VII (15 isolates) specifically existed in South America. These results suggest that the population genetic structure of C. kikuchii was different between South America and Japan, but the dominance of lineage I was common between the two areas.