July
2001
, Volume
14
, Number
7
Pages
905
-
909
Authors
R. A.
Owens
,
1
M.
Blackburn
,
2
and
B.
Ding
3
Affiliations
1Molecular Plant Pathology Laboratory, USDA-ARS, Beltsville, MD 20705, U.S.A.; 2Insect Biocontrol Laboratory, USDA-ARS, Beltsville, MD 20705, U.S.A.; 3Department of Plant Biology and Plant Biotechnology Center, Ohio State University, Columbus 43210, U.S.A.
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RelatedArticle
Accepted 19 March 2001.
Abstract
Incubation with cucumber phloem exudate in vitro results in a dramatic decrease in the electrophoretic mobility of Hop stunt viroid. UV cross-linking and a combination of size exclusion and ion exchange chromatography indicate that this phenomenon reflects a previously unsuspected ability of phloem protein 2, a dimeric lectin and the most abundant component of phloem exudate, to interact with RNA. In light of its demonstrated ability to move from cell to cell via plasmodesmata as well as long distances in the phloem, our results suggest that phloem protein 2 may facilitate the systemic movement of viroids and, possibly, other RNAs in vivo.
JnArticleKeywords
Additional keyword:
RNA-binding protein.
Page Content
ArticleCopyright
The American Phytopathological Society, 2001
