March
2001
, Volume
14
, Number
3
Pages
422
-
425
Authors
Artem E.
Men
,
1
,
2
Khalid
Meksem
,
3
My Abdelmajid
Kassem
,
3
Dasharath
Lohar
,
2
Jiri
Stiller
,
1
,
2
David
Lightfoot
,
3
and
Peter M.
Gresshoff
1
Affiliations
1Department of Botany, University of Queensland, Brisbane, QLD 4072, Australia; 2Plant Molecular Genetics, University of Tennessee, Knoxville 37996-4500, U.S.A.; 3Department of Plant Soil and General Agriculture, Southern Illinois University, Carbondale 62901-4415, U.S.A.
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RelatedArticle
Accepted 28 November 2000.
Abstract
We constructed a BAC library of the model legume Lotus japonicus with a 6-to 7-fold genome coverage. We used vector PCLD04541, which allows direct plant transformation by BACs. The average insert size is 94 kb. Clones were stable in Escherichia coli and Agrobacterium tumefaciens.
JnArticleKeywords
Additional keywords:
Arabidopsis,
functional genomics,
map-based cloning,
symbiosis.
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ArticleCopyright
© 2001 The American Phytopathological Society
