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Genetic Variation and Virulence on Lr26 in Puccinia triticina

First, third, fourth, fifth, and seventh authors: Institute for Cereal Crops Improvement,; and second, third, and seventh authors: The Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel; and sixth author: Cereal Disease Laboratory, Agricultural Research Service, U.S. Department of Agriculture, St. Paul, MN 55108

The genetic relationships between isolates of Puccinia triticina virulent on wheat with the Lr26 resistance gene were studied. The diversity within and between isolates of P. triticina from Israel, Europe, and the United States was determined by virulence on near-isogenic Thatcher lines and by random amplified polymorphic DNA. According to the molecular markers, isolates that were virulent on Lr26 had diversity levels similar to those of Lr26 nonpathogenic isolates. Distances between subpopulations of isolates virulent and avirulent on Lr26 varied and were unrelated to the Lr26 virulence phenotype. Cluster analysis suggested four groups, three of which were closely associated with the geographical origin of the isolates—Israel, the United States, and Europe. All four groups included both Lr26 virulent and avirulent pathotypes. The results showed that Lr26 virulent rust pathotypes are as genetically dissimilar as the rest of the population. The cluster analysis showed that the rust population in Israel includes at least two different subpopulations, both of which contain Lr26 virulent and Lr26 avirulent isolates.

Additional keywords: Kosman index, rust virulence genes.