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Soybean Root Suberin and Partial Resistance to Root Rot Caused by Phytophthora sojae

First, third, and fourth authors: Department of Biology, University of Waterloo, Waterloo, ON, Canada, N2L 3G1; second and sixth authors: Environmental Stress Biology Group, Department of Biology, University of Western Ontario, London, ON, Canada, N6A 5B7; and fifth author: Agriculture and Agri-Food Canada, London, ON, Canada, N5V 4T3.

Phytophthora sojae is the causal agent of root and stem rot of soybean (Glycine max). Various cultivars with partial resistance to the pathogen have been developed to mitigate this damage. Herein, two contrasting genotypes, the cultivar Conrad (with strong partial resistance) and the line OX760-6 (with weak partial resistance), were compared regarding their amounts of preformed and induced suberin components, and to early events during the P. sojae infection process. To colonize the root, hyphae grew through the suberized middle lamellae between epidermal cells. This took 2 to 3 h longer in Conrad than in OX760-6, giving Conrad plants more time to establish their chemical defenses. Subsequent growth of hyphae through the endodermis was also delayed in Conrad. This cultivar had more preformed aliphatic suberin than the line OX760-6 and was induced to form more aliphatic suberin several days prior to that of OX760-6. However, the induced suberin was formed subsequent to the initial infection process. Eventually, the amount of induced suberin (measured 8 days postinoculation) was the same in both genotypes. Preformed root epidermal suberin provides a target for selection and development of new soybean cultivars with higher levels of expression of partial resistance to P. sojae.

Additional keywords:oomycete hyphal staining, preformed defenses, root epidermis.