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Proteomic Analysis Reveals Suppression of Bark Chitinases and Proteinase Inhibitors in Citrus Plants Affected by the Citrus Sudden Death Disease

First and fourth authors: Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil; second and fifth authors: Fundecitrus, Fundo de Defesa da Citricultura, Araraquara, SP, Brazil; and third author: Instituto de Biociências de Rio Claro, Universidade Estadual Paulista, Rio Claro, SP, Brazil.

Citrus sudden death (CSD) is a disease of unknown etiology that greatly affects sweet oranges grafted on Rangpur lime rootstock, the most important rootstock in Brazilian citriculture. We performed a proteomic analysis to generate information related to this plant pathogen interaction. Protein profiles from healthy, CSD-affected and CSD-tolerant stem barks, were generated using two-dimensional gel electrophoresis. The protein spots were well distributed over a pI range of 3.26 to 9.97 and a molecular weight (MW) range from 7.1 to 120 kDa. The patterns of expressed proteins on 2-DE gels made it possible to distinguish healthy barks from CSD-affected barks. Protein spots with MW around 30 kDa and pI values ranging from 4.5 to 5.2 were down-regulated in the CSD-affected root-stock bark. This set of protein spots was identified as chitinases. Another set of proteins, ranging in pI from 6.1 to 9.6 with an MW of about 20 kDa, were also suppressed in CSD-affected rootstock bark; these were identified as miraculin-like proteins, potential trypsin inhibitors. Down-regulation of chitinases and proteinase inhibitors in CSD-affected plants is relevant since chitinases are well-known pathogenesis-related protein, and their activity against plant pathogens is largely accepted.

Additional keywords:plant proteomics.