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Molecular, Ultrastructural, and Biological Characterization of Pennsylvania Isolates of Plum pox virus

First, second, fourth, fifth, and tenth authors: United States Department of Agriculture (USDA) Agricultural Research Service, Foreign Disease-Weed Science Research Unit, 1301 Ditto Ave., Ft. Detrick, MD 21702; third author: Department of Plant Pathology, Penn State University, Buckhout Laboratory, University Park, PA 16802-5023; sixth and seventh authors: USDA Animal and Plant Health Inspection Service, PPQ-CPHST National Plant Germplasm and Biotechnology Laboratory, Beltsville, MD 20705; and eighth and ninth authors: Pennsylvania Department of Agriculture, Harrisburg.

Plum pox virus (PPV) was identified in Pennsylvania in 1999. The outbreak was limited to a four-county region in southern Pennsylvania. Initial serological and molecular characterization indicated that the isolates in Pennsylvania belong to the D strain of PPV. The Pennsylvania isolates were characterized by sequence analysis, electron microscopy, host range, and vector transmission to determine how these isolates related to their previously studied European counterparts. Genetically, Pennsylvania (PPV-Penn) isolates were more closely related to each other than to any other PPV-D strains, and isolates from the United States, Canada, and Chile were more closely related to each other than to European isolates. The PPV-Penn isolates exist as two clades, suggesting the possibility of multiple introductions. Electron microscopy analysis of PPV-Penn isolates, including cytopathological studies, indicated that the virions were similar to other Potyvirus spp. PPV-Penn isolates had a herbaceous host range similar to that of European D isolates. There were distinct differences in the transmission efficiencies of the two PPV-Penn isolates using Myzus persicae and Aphis spiraecola as vectors; however, both PPV-Penn isolates were transmitted by M. persicae more efficiently than a European D isolate but less efficiently than a European M isolate.