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Differential Responses of Three Grapevine Cultivars to Botryosphaeria Dieback

First, second, seventh, and thirteenth authors: Université de Reims Champagne-Ardenne, URVVC EA 4707, Laboratoire Stress, Défenses et Reproduction des Plantes, BP 1039, 51687 Reims Cedex 2, France; third, fifth, and sixth authors: Université de Strasbourg, IPHC, UMR 7178, Laboratoire de Spectrométrie de Masse Bioorganique, 67087 Strasbourg, France; fourth author: Université de Reims Champagne-Ardenne, URVVC EA 4707, Laboratoire d'Œnologie et Chimie Appliquée, BP 1039, 51687 Reims Cedex 2, France; eighth author: Institut Français de la Vigne et du Vin Pôle Rhône-Méditerranée, France, Domaine de Donadille, 30230 Rodilhan, France; ninth, tenth, and eleventh authors: Université de Haute-Alsace, UFR PEPS, Laboratoire Vigne, Biotechnologie et Environnement, 33 rue de Herrlisheim, 68008 Colmar cedex, France; and twelfth author: Plant Biology Department, University of Fribourg, 3 rue Albert Gockel, 1700 Fribourg, Switzerland.

Botryosphaeria dieback is a fungal grapevine trunk disease that represents a threat for viticulture worldwide due to the decreased production of affected plants and their premature death. This dieback is characterized by a typical wood discoloration called brown stripe. Herein, a proteome comparison of the brown striped wood from Botryosphaeria dieback-affected standing vines cultivars Chardonnay, Gewurztraminer, and Mourvèdre was performed. The transcript analysis for 15 targeted genes and the quantification of both total phenolics and specific stilbenes were also performed. Several pathogenesis-related proteins and members of the antioxidant system were more abundant in the brown striped wood of the three cultivars, whereas other defense-related proteins were less abundant. Additionally, total phenolics and some specific stilbenes were more accumulated in the brown striped wood. Strongest differences among the cultivars concerned proteins of the primary metabolism, which looked to be particularly impaired in the brown striped wood of ‘Chardonnay’. Low abundance of some proteins involved in defense response probably contributes to make global response insufficient to avoid the symptom development. The differential susceptibility of the three grapevine cultivars could be linked to the diverse expression of various proteins involved in defense response, stress tolerance, and metabolism.

Additional keywords: two-dimensional gel electrophoresis, phytoalexins.