September
2001
, Volume
85
, Number
9
Pages
989
-
992
Authors
Ralf G.
Dietzgen
,
Queensland Government, Department of Primary Industries, Queensland Agricultural Biotechnology Centre, Gehrmann Laboratories, The University of Queensland, St. Lucia Qld 4072, Australia
;
Ben
Callaghan
,
Queensland Government, Department of Primary Industries, Queensland Agricultural Biotechnology Centre, Gehrmann Laboratories, The University of Queensland, St. Lucia Qld 4072, Australia; and Botany Department, The University of Queensland, St. Lucia Qld 4072, Australia
;
Colleen M.
Higgins
,
Queensland Government, Department of Primary Industries, Queensland Agricultural Biotechnology Centre, Gehrmann Laboratories, The University of Queensland, St. Lucia Qld 4072, Australia
;
Robert G.
Birch
,
Botany Department, The University of Queensland, St. Lucia Qld 4072, Australia
;
Kunrong
Chen
and
Zeyong
Xu
,
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, 430062 Wuhan, China
Affiliations
Go to article:
RelatedArticle
Accepted for publication 31 May 2001.
Abstract
ABSTRACT
Seedborne peanut viruses pose important constraints to peanut production and safe movement of germ plasm. They also pose a risk of accidental introduction into previously disease-free regions. We have developed reverse transcription-polymerase chain reaction (RT-PCR) assays based on identical cycling parameters which identified peanut stripe, Peanut mottle, Peanut stunt, and Cucumber mosaic viruses through production of specific DNA fragments of 234 bp, 327 bp, 390 bp, and 133 bp, respectively. Assay sensitivity in the picogram range was achieved. The two potyviruses and two cucumoviruses could be differentiated using duplex RT-PCR assays. These assays should be useful for testing peanut leaves or seeds for virus identification in epidemiological studies, seed testing or in post-entry quarantine.
JnArticleKeywords
Additional keywords:
duplex RT-PCR,
groundnut,
virus diagnosis
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ArticleCopyright
© 2001 The American Phytopathological Society
