January
2003
, Volume
87
, Number
1
Pages
33
-
38
Authors
M.
Delanoy
,
M.
Salmon
, and
J.
Kummert
,
Plant Pathology Unit, Gembloux Agricultural University, 5030 Gembloux, Belgium
;
E.
Frison
,
International Network for the Improvement of Banana and Plantain/International Plant Genetic Resources Institute (INIBAP/IPGRI), 34397 Montpellier, France
; and
P.
Lepoivre
,
Plant Pathology Unit, Gembloux Agricultural University, 5030 Gembloux, Belgium
Affiliations
Go to article:
RelatedArticle
Accepted for publication 16 August 2002.
Abstract
ABSTRACT
A real-time assay for the detection of episomal Banana streak virus (BSV; strain OL) in banana and plantains that carry integrated BSV sequences is described. Primers specific to the viral DNA were designed using the viral sequence integrated into the cv. Obino l'Ewai genome and the sequence of the genomic DNA of the infecting virus strain OL. They amplify a sequence of 1,336 bp that is detected in real-time by a short fluorogenic 3' minor groove binder DNA probe. This method enables reproducible and specific detection of episomal BSV from purified DNA as well as from crude extracts from infected plants. The assay is rapid, adaptable for large-scale experiments, and circumvents carryover problems.
JnArticleKeywords
Additional keywords:
badnavirus,
long amplicon,
minor groove binder (MGB),
Musa,
5′ nuclease assay
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ArticleCopyright
© 2003 The American Phytopathological Society
